Detailed information of TA system TA034420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 159169..159781
• Replicon: chromosome
• Accession: NZ_AP022087
• Organism: Escherichia coli strain WP4-S18-ESBL-07

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: H7R53_RS00685
• Protein ID: WP_000833473.1
• Coordinates: 159169..159354 (+)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: H7R53_RS00690
• Protein ID: WP_000499744.1
• Coordinates: 159371..159781 (+)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R53_RS00670	154686..155837	+	1152	WP_000741506.1	L-threonine dehydrogenase	-
H7R53_RS00675	156038..157576	+	1539	WP_001469350.1	aldehyde dehydrogenase AldB	-
H7R53_RS00680	157617..158696	-	1080	WP_000061477.1	type I restriction enzyme HsdR N-terminal domain-containing protein	-
H7R53_RS00685	159169..159354	+	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
H7R53_RS00690	159371..159781	+	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
H7R53_RS00695	159853..161817	-	1965	WP_024216364.1	glycoside hydrolase family 127 protein	-
H7R53_RS00700	161828..163228	-	1401	WP_024216365.1	MFS transporter	-
H7R53_RS00705	163454..164269	+	816	WP_000891838.1	AraC family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T34420 WP_000833473.1 NZ_AP022087:159169-159354 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T34420 NZ_AP022087:159169-159354 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT34420 WP_000499744.1 NZ_AP022087:159371-159781 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT34420 NZ_AP022087:159371-159781 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCAAAAAGATTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTATGCCAACAGAAGTGCGTTTCTGGCGGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9