Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 110133..110658 Replicon plasmid pWP4-S18-CRE-04_1
Accession NZ_AP022079
Organism Klebsiella pneumoniae strain WP4-S18-CRE-04

Toxin (Protein)


Gene name ccdB Uniprot ID J5VBT8
Locus tag H7R40_RS26780 Protein ID WP_004197633.1
Coordinates 110353..110658 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A1D8K3R4
Locus tag H7R40_RS26775 Protein ID WP_006788213.1
Coordinates 110133..110351 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R40_RS26755 106035..107312 - 1278 WP_048321652.1 HlyD family secretion protein -
H7R40_RS26760 107974..108852 + 879 WP_032427228.1 restriction endonuclease -
H7R40_RS26765 108893..109309 - 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin -
H7R40_RS26770 109306..109536 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
H7R40_RS26775 110133..110351 + 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H7R40_RS26780 110353..110658 + 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB Toxin
H7R40_RS26785 110887..111027 + 141 WP_162898808.1 hypothetical protein -
H7R40_RS26790 111077..111412 + 336 WP_009309920.1 hypothetical protein -
H7R40_RS26795 111446..112462 + 1017 WP_009309921.1 hypothetical protein -
H7R40_RS26800 112660..113439 + 780 WP_023287113.1 site-specific integrase -
H7R40_RS26805 113497..113754 - 258 WP_009310077.1 hypothetical protein -
H7R40_RS26810 113883..113996 - 114 WP_014343462.1 hypothetical protein -
H7R40_RS26815 114099..115079 + 981 WP_014386491.1 IS5-like element ISKpn26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..224088 224088


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11585.27 Da        Isoelectric Point: 6.4661

>T34418 WP_004197633.1 NZ_AP022079:110353-110658 [Klebsiella pneumoniae]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T34418 NZ_AP022079:110353-110658 [Klebsiella pneumoniae]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGACAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8362.31 Da        Isoelectric Point: 4.7405

>AT34418 WP_006788213.1 NZ_AP022079:110133-110351 [Klebsiella pneumoniae]
MKQRITVTIDSDSYQLLKSADVNISGLVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT34418 NZ_AP022079:110133-110351 [Klebsiella pneumoniae]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGGCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCCGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MCG5

Antitoxin

Source ID Structure
AlphaFold DB A0A1D8K3R4

References