Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 108893..109536 Replicon plasmid pWP4-S18-CRE-04_1
Accession NZ_AP022079
Organism Klebsiella pneumoniae strain WP4-S18-CRE-04

Toxin (Protein)


Gene name vagD Uniprot ID A0A3Q8D6L7
Locus tag H7R40_RS26765 Protein ID WP_020804312.1
Coordinates 108893..109309 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag H7R40_RS26770 Protein ID WP_001261276.1
Coordinates 109306..109536 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R40_RS26750 103937..106045 - 2109 WP_032427226.1 peptidase domain-containing ABC transporter -
H7R40_RS26755 106035..107312 - 1278 WP_048321652.1 HlyD family secretion protein -
H7R40_RS26760 107974..108852 + 879 WP_032427228.1 restriction endonuclease -
H7R40_RS26765 108893..109309 - 417 WP_020804312.1 type II toxin-antitoxin system VapC family toxin Toxin
H7R40_RS26770 109306..109536 - 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H7R40_RS26775 110133..110351 + 219 WP_006788213.1 type II toxin-antitoxin system antitoxin CcdA -
H7R40_RS26780 110353..110658 + 306 WP_004197633.1 type II toxin-antitoxin system toxin CcdB -
H7R40_RS26785 110887..111027 + 141 WP_162898808.1 hypothetical protein -
H7R40_RS26790 111077..111412 + 336 WP_009309920.1 hypothetical protein -
H7R40_RS26795 111446..112462 + 1017 WP_009309921.1 hypothetical protein -
H7R40_RS26800 112660..113439 + 780 WP_023287113.1 site-specific integrase -
H7R40_RS26805 113497..113754 - 258 WP_009310077.1 hypothetical protein -
H7R40_RS26810 113883..113996 - 114 WP_014343462.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..224088 224088


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15154.59 Da        Isoelectric Point: 7.8637

>T34417 WP_020804312.1 NZ_AP022079:c109309-108893 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPESVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T34417 NZ_AP022079:c109309-108893 [Klebsiella pneumoniae]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGAATCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGACCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT34417 WP_001261276.1 NZ_AP022079:c109536-109306 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT34417 NZ_AP022079:c109536-109306 [Klebsiella pneumoniae]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATTCGGCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3Q8D6L7

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References