Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 99414..100057 Replicon plasmid pWP4-S18-CRE-04_1
Accession NZ_AP022079
Organism Klebsiella pneumoniae strain WP4-S18-CRE-04

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag H7R40_RS26710 Protein ID WP_001044770.1
Coordinates 99414..99830 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag H7R40_RS26715 Protein ID WP_001261282.1
Coordinates 99827..100057 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R40_RS26690 94783..95133 + 351 WP_004187110.1 DUF305 domain-containing protein -
H7R40_RS26695 95319..96233 - 915 WP_031592169.1 HNH endonuclease -
H7R40_RS26700 96771..97793 - 1023 WP_000361404.1 helicase UvrD -
H7R40_RS26705 97778..99340 - 1563 WP_009309907.1 AAA family ATPase -
H7R40_RS26710 99414..99830 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
H7R40_RS26715 99827..100057 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H7R40_RS26720 100014..100250 + 237 Protein_99 hypothetical protein -
H7R40_RS26725 100349..100942 + 594 WP_182920197.1 hypothetical protein -
H7R40_RS26730 100961..101401 + 441 WP_047057603.1 hypothetical protein -
H7R40_RS26735 101592..102528 - 937 Protein_102 CAAX protease -
H7R40_RS26740 102850..103194 + 345 WP_009309911.1 hypothetical protein -
H7R40_RS26745 103302..103937 - 636 WP_009309912.1 CPBP family intramembrane metalloprotease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..224088 224088


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T34416 WP_001044770.1 NZ_AP022079:c99830-99414 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T34416 NZ_AP022079:c99830-99414 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT34416 WP_001261282.1 NZ_AP022079:c100057-99827 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT34416 NZ_AP022079:c100057-99827 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References