Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5283105..5283730 Replicon chromosome
Accession NZ_AP022078
Organism Klebsiella pneumoniae strain WP4-S18-CRE-04

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag H7R40_RS25860 Protein ID WP_019705794.1
Coordinates 5283105..5283488 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag H7R40_RS25865 Protein ID WP_004150355.1
Coordinates 5283488..5283730 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R40_RS25845 5280471..5281373 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
H7R40_RS25850 5281370..5282005 + 636 WP_032430299.1 formate dehydrogenase cytochrome b556 subunit -
H7R40_RS25855 5282002..5282931 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
H7R40_RS25860 5283105..5283488 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
H7R40_RS25865 5283488..5283730 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
H7R40_RS25870 5283935..5284852 + 918 WP_032430300.1 alpha/beta hydrolase -
H7R40_RS25875 5284867..5285808 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
H7R40_RS25880 5285853..5286290 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
H7R40_RS25885 5286287..5287147 - 861 WP_004146232.1 virulence factor BrkB family protein -
H7R40_RS25890 5287141..5287740 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T34414 WP_019705794.1 NZ_AP022078:c5283488-5283105 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T34414 NZ_AP022078:c5283488-5283105 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCATCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT34414 WP_004150355.1 NZ_AP022078:c5283730-5283488 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT34414 NZ_AP022078:c5283730-5283488 [Klebsiella pneumoniae]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References