Detailed information of TA system TA034395

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-couple_hipB
• Location: 2028990..2030626
• Replicon: chromosome
• Accession: NZ_AP022077
• Organism: Acinetobacter baumannii strain WP4-W18-ESBL-11

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A5K1MIT9
• Locus tag: H7K53_RS09785
• Protein ID: WP_004739062.1
• Coordinates: 2029295..2030626 (-)
• Length: 444 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: H7K53_RS09780
• Protein ID: WP_004739059.1
• Coordinates: 2028990..2029298 (-)
• Length: 103 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7K53_RS09755	2024561..2025586	+	1026	WP_004994718.1	IS30-like element ISAba125 family transposase	-
H7K53_RS09760	2025577..2026158	-	582	WP_182638710.1	hypothetical protein	-
H7K53_RS09765	2026231..2026797	-	567	WP_171491485.1	TetR/AcrR family transcriptional regulator	-
H7K53_RS09770	2027221..2027729	+	509	Protein_1908	transposase	-
H7K53_RS09775	2027705..2028604	+	900	Protein_1909	IS3 family transposase	-
H7K53_RS09780	2028990..2029298	-	309	WP_004739059.1	helix-turn-helix domain-containing protein	Antitoxin
H7K53_RS09785	2029295..2030626	-	1332	WP_004739062.1	type II toxin-antitoxin system HipA family toxin	Toxin
H7K53_RS09790	2030922..2031362	+	441	WP_004739065.1	HD domain-containing protein	-
H7K53_RS09795	2031398..2032042	-	645	WP_004739067.1	hypothetical protein	-
H7K53_RS09800	2032054..2033025	-	972	WP_109104323.1	5'/3'-nucleotidase SurE	-
H7K53_RS09805	2033150..2034313	-	1164	WP_004739071.1	hypothetical protein	-
H7K53_RS09810	2034306..2035544	-	1239	WP_109104325.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2021291..2058609	37318

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (167-359)

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-83)

Sequences

Toxin

Protein

• Length: 444 a.a.
• Molecular weight: 51203.32 Da
• Isoelectric Point: 4.9177

>T34395 WP_004739062.1 NZ_AP022077:c2030626-2029295 [Acinetobacter baumannii]
MKKLTIQALLNKNWIDIAELKLLEPKLGSASASELEYELDYAIQYLDRRDEHACSLSLPVQILIKHESDTWFGFLDDIVP
SGAARRYWVNYLDLQRLTYAEQESILLEKGGMAPVGNLRIKEALPPLNPESTLHLRYFSKEDVAERNIDFLEYAQQMGAI
SGGATGAGGEAPKLLIRVSPEQKVWIDTYQEKFDQPDQHYLVKFPRNKRSEIDCDILRAEYYYYQILNELGFNTIETTQM
KLIEGTKYPSLWLPRFDTEWCDQYWHRYGLESVYSVLNKSSGSHLNHFEVIEDLCRLLTSIDSDFDVPQFICEWLQRDLL
NIIFGNSDNHGRNTSFIKKSGKIYLSPIYDFAPMKADPEVVTRSTTWGSPYEEGGEYRWAQITQKLAPWCDPDLSLATLR
TLANNLLGLKQRLVDKGVPKQIIEMPALSFDYIEAKLKRWELL

Nucleotide

• Length: 1332 bp

>T34395 NZ_AP022077:c2030626-2029295 [Acinetobacter baumannii]
ATGAAAAAATTAACGATACAGGCTTTACTAAATAAAAACTGGATAGATATAGCAGAACTAAAACTGCTGGAACCTAAGTT
GGGTTCGGCTAGCGCCAGTGAACTAGAATATGAATTGGATTATGCAATTCAATATTTAGACAGACGAGATGAGCACGCCT
GTAGTTTATCACTTCCTGTACAAATTCTTATCAAACACGAATCGGACACATGGTTTGGCTTTTTAGATGATATTGTTCCA
TCTGGCGCTGCACGCCGTTATTGGGTGAATTATCTAGATTTACAACGCTTAACCTATGCGGAACAAGAAAGTATTCTATT
AGAAAAAGGTGGGATGGCACCTGTTGGTAATTTACGAATTAAAGAAGCATTGCCTCCACTAAACCCAGAGTCAACCTTGC
ACTTACGTTATTTTAGTAAGGAAGATGTGGCTGAGCGTAATATTGATTTTCTGGAATATGCTCAACAAATGGGAGCTATT
AGTGGTGGTGCAACTGGTGCTGGCGGGGAAGCTCCCAAATTACTTATTCGTGTTTCACCGGAACAAAAAGTCTGGATTGA
CACCTACCAAGAAAAATTTGATCAGCCAGATCAGCATTATTTGGTGAAATTCCCGCGAAATAAACGTAGCGAGATTGATT
GCGATATTCTCAGAGCCGAATATTATTATTATCAGATACTCAATGAGTTAGGTTTCAACACAATTGAAACAACCCAAATG
AAGCTCATTGAAGGTACAAAATATCCGTCATTGTGGCTTCCAAGATTCGATACAGAATGGTGTGATCAATATTGGCACCG
CTATGGTCTAGAGTCTGTGTATTCTGTACTAAATAAGTCTTCTGGTAGTCATCTTAATCATTTCGAAGTCATTGAAGATC
TGTGTAGATTACTCACAAGCATTGACTCAGATTTTGATGTACCACAATTTATTTGTGAGTGGTTACAACGCGACTTACTC
AATATCATTTTTGGCAATTCAGATAACCACGGTCGGAATACATCGTTTATAAAAAAATCTGGAAAAATTTATCTTTCGCC
TATTTATGACTTTGCACCTATGAAAGCCGATCCTGAAGTTGTGACCAGATCGACGACTTGGGGAAGTCCGTATGAGGAAG
GAGGTGAATACCGGTGGGCGCAAATTACACAAAAACTTGCTCCTTGGTGCGATCCCGATTTATCTCTAGCAACATTAAGG
ACTTTGGCGAATAACCTGCTTGGCTTAAAGCAACGATTAGTTGATAAAGGTGTACCCAAACAAATTATTGAAATGCCAGC
GCTGTCATTTGATTACATAGAAGCTAAATTGAAAAGATGGGAACTGCTATGA

Antitoxin

Protein

• Length: 103 a.a.
• Molecular weight: 11616.51 Da
• Isoelectric Point: 9.9701

>AT34395 WP_004739059.1 NZ_AP022077:c2029298-2028990 [Acinetobacter baumannii]
MNVKNQSQDRQQILIDLYKQYLLSEITLGQLLSYLRKNVLGLSQEQYANLVGISRRTLTDIEQDKGKLTQSVLDKVFKPL
GLKAGLVPTHEHIVSKIIKPNE

Nucleotide

• Length: 309 bp

>AT34395 NZ_AP022077:c2029298-2028990 [Acinetobacter baumannii]
ATGAATGTAAAAAATCAATCCCAAGACCGTCAGCAGATTCTTATAGATTTATATAAACAATATTTGTTGAGTGAGATTAC
ATTGGGGCAACTGCTATCGTATTTACGCAAAAATGTGTTGGGTTTGTCGCAAGAACAATATGCTAATTTGGTGGGTATAA
GCCGTCGGACTTTAACGGACATTGAGCAGGATAAAGGTAAACTCACTCAGTCTGTGTTAGATAAAGTGTTTAAACCCCTT
GGTTTAAAAGCAGGGCTTGTACCTACGCATGAACATATTGTTAGCAAAATTATAAAGCCAAACGAGTAA

Structures

Toxin

• AlphaFold DB: A0A5K1MIT9