Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 60658..61183 Replicon plasmid pWP4-W18-ESBL-09_3
Accession NZ_AP022072
Organism Escherichia coli strain WP4-W18-ESBL-09

Toxin (Protein)


Gene name ccdB Uniprot ID S1PFV8
Locus tag H7R28_RS26770 Protein ID WP_001159871.1
Coordinates 60878..61183 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A4D9F4P3
Locus tag H7R28_RS26765 Protein ID WP_032256270.1
Coordinates 60658..60876 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R28_RS26730 55738..56019 + 282 WP_000970000.1 type II toxin-antitoxin system RelE/ParE family toxin -
H7R28_RS26735 56065..56913 + 849 WP_032256585.1 3'-5' exonuclease -
H7R28_RS26740 57030..57512 + 483 WP_001311056.1 hypothetical protein -
H7R28_RS26745 57814..58161 + 348 WP_088251382.1 hypothetical protein -
H7R28_RS26750 58179..58769 - 591 WP_032256264.1 hypothetical protein -
H7R28_RS26755 58993..59178 + 186 WP_032256266.1 hypothetical protein -
H7R28_RS26760 59241..60110 - 870 WP_032256267.1 hypothetical protein -
H7R28_RS26765 60658..60876 + 219 WP_032256270.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H7R28_RS26770 60878..61183 + 306 WP_001159871.1 type II toxin-antitoxin system toxin CcdB Toxin
H7R28_RS26775 61505..62639 - 1135 Protein_80 IS91 family transposase -
H7R28_RS26780 62602..63003 - 402 WP_050488175.1 hypothetical protein -
H7R28_RS26785 63531..64040 + 510 WP_000115001.1 peptide deformylase -
H7R28_RS26790 64055..64156 + 102 Protein_83 methionyl-tRNA formyltransferase -
H7R28_RS26795 64416..64544 + 129 Protein_84 DUF2164 family protein -
H7R28_RS26800 64541..64903 + 363 WP_000181069.1 hypothetical protein -
H7R28_RS26805 65236..65763 - 528 WP_001196880.1 DoxX family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..77891 77891
- flank IS/Tn - - 61505..62359 854


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11692.49 Da        Isoelectric Point: 6.4674

>T34394 WP_001159871.1 NZ_AP022072:60878-61183 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T34394 NZ_AP022072:60878-61183 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTTCAGAGTGACATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCCCGCCTGCTGTCAGACAAAGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGTCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8388.34 Da        Isoelectric Point: 4.7405

>AT34394 WP_032256270.1 NZ_AP022072:60658-60876 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMSEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT34394 NZ_AP022072:60658-60876 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAATTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGTCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGATGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CCE8

Antitoxin

Source ID Structure
AlphaFold DB A0A4D9F4P3

References