Detailed information of TA system TA034381

Bioinformatically predicted

Overview

TA module

• Type: V
• Classification (family/domain): ghoTS/ghoT-GhoS
• Location: 4803690..4804187
• Replicon: chromosome
• Accession: NZ_AP022069
• Organism: Escherichia coli strain WP4-W18-ESBL-09

Toxin (Protein)

• Gene name: ghoT
• Uniprot ID: -
• Locus tag: H7R28_RS23015
• Protein ID: WP_182918124.1
• Coordinates: 4803690..4803863 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: ghoS
• Uniprot ID: L4JK92
• Locus tag: H7R28_RS23020
• Protein ID: WP_000398619.1
• Coordinates: 4803891..4804187 (-)
• Length: 99 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R28_RS23005	4800360..4801817	+	1458	WP_000856827.1	dipeptide/tripeptide permease DtpC	-
H7R28_RS23010	4802054..4803571	+	1518	WP_000003806.1	lysine--tRNA ligase	-
H7R28_RS23015	4803690..4803863	-	174	WP_182918124.1	type V toxin-antitoxin system toxin GhoT	Toxin
H7R28_RS23020	4803891..4804187	-	297	WP_000398619.1	type V toxin-antitoxin system endoribonuclease antitoxin GhoS	Antitoxin
H7R28_RS23025	4804416..4804688	-	273	WP_000405647.1	N-acetyltransferase	-
H7R28_RS23030	4804700..4804930	-	231	WP_000373291.1	(4Fe-4S)-binding protein	-
H7R28_RS23035	4805111..4806742	+	1632	WP_001216477.1	sensor histidine kinase	-
H7R28_RS23040	4806739..4807458	+	720	WP_000611288.1	two-component system response regulator DcuR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4758407..4804187	45780

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin GhoT/OrtT (4-57)

Antitoxin

• Endoribonuclease antitoxin GhoS (5-94)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6583.12 Da
• Isoelectric Point: 10.2288

>T34381 WP_182918124.1 NZ_AP022069:c4803863-4803690 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVVLLFSLF

Nucleotide

• Length: 174 bp

>T34381 NZ_AP022069:c4803863-4803690 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGTATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 99 a.a.
• Molecular weight: 11467.97 Da
• Isoelectric Point: 4.1705

>AT34381 WP_000398619.1 NZ_AP022069:c4804187-4803891 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Nucleotide

• Length: 297 bp

>AT34381 NZ_AP022069:c4804187-4803891 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAATT
CCTTCGCAATAACGACATCATTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTCAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• PDB: 2LLZ
• AlphaFold DB: A0A7U9QBH6