Detailed information of TA system TA034375

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4296876..4297363
• Replicon: chromosome
• Accession: NZ_AP022069
• Organism: Escherichia coli strain WP4-W18-ESBL-09

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: H7R28_RS20475
• Protein ID: WP_001520461.1
• Coordinates: 4296876..4297127 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A2T1MJK4
• Locus tag: H7R28_RS20480
• Protein ID: WP_001520458.1
• Coordinates: 4297130..4297363 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R28_RS20450	4292488..4293774	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
H7R28_RS20455	4293774..4294763	+	990	WP_001520462.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
H7R28_RS20460	4294760..4295581	+	822	WP_001065381.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
H7R28_RS20465	4295584..4295961	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
H7R28_RS20470	4295968..4296816	+	849	WP_000257196.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
H7R28_RS20475	4296876..4297127	-	252	WP_001520461.1	CcdB family protein	Toxin
H7R28_RS20480	4297130..4297363	-	234	WP_001520458.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
H7R28_RS20485	4297449..4297928	-	480	WP_000624375.1	type 3 dihydrofolate reductase	-
H7R28_RS20490	4298120..4299982	-	1863	WP_000377113.1	glutathione-regulated potassium-efflux system protein KefC	-
H7R28_RS20495	4299975..4300505	-	531	WP_021522993.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
H7R28_RS20500	4300613..4301944	-	1332	WP_001520455.1	MFS transporter	-
H7R28_RS20505	4302003..4302290	-	288	WP_000203747.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-82)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9482.12 Da
• Isoelectric Point: 10.4134

>T34375 WP_001520461.1 NZ_AP022069:c4297127-4296876 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKF

Nucleotide

• Length: 252 bp

>T34375 NZ_AP022069:c4297127-4296876 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATTCTTTTACTGGTAGATGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8560.61 Da
• Isoelectric Point: 5.8062

>AT34375 WP_001520458.1 NZ_AP022069:c4297363-4297130 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLMVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT34375 NZ_AP022069:c4297363-4297130 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTATGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Structures

Antitoxin

• AlphaFold DB: A0A2T1MJK4