Detailed information of TA system TA034368

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2551782..2552420
• Replicon: chromosome
• Accession: NZ_AP022069
• Organism: Escherichia coli strain WP4-W18-ESBL-09

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A0D8WF76
• Locus tag: H7R28_RS12085
• Protein ID: WP_001306887.1
• Coordinates: 2551782..2551958 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H7R28_RS12090
• Protein ID: WP_001270286.1
• Coordinates: 2552004..2552420 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R28_RS12060	2546839..2547296	+	458	Protein_2360	IS200/IS605 family transposase	-
H7R28_RS12065	2547404..2548615	-	1212	WP_023143671.1	BenE family transporter YdcO	-
H7R28_RS12070	2548668..2549204	+	537	WP_020233877.1	DNA-binding transcriptional regulator SutR	-
H7R28_RS12075	2549277..2551238	+	1962	WP_024166512.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
H7R28_RS12080	2551330..2551560	-	231	WP_000494241.1	YncJ family protein	-
H7R28_RS12085	2551782..2551958	+	177	WP_001306887.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
H7R28_RS12090	2552004..2552420	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
H7R28_RS12095	2552499..2553905	+	1407	WP_001523229.1	PLP-dependent aminotransferase family protein	-
H7R28_RS12100	2554150..2555295	+	1146	WP_020233879.1	ABC transporter substrate-binding protein	-
H7R28_RS12105	2555313..2556326	+	1014	WP_001523231.1	ABC transporter ATP-binding protein	-
H7R28_RS12110	2556327..2557268	+	942	WP_001251315.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2547051..2547296	245

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6768.88 Da
• Isoelectric Point: 11.5336

>T34368 WP_001306887.1 NZ_AP022069:2551782-2551958 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T34368 NZ_AP022069:2551782-2551958 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCGTGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT34368 WP_001270286.1 NZ_AP022069:2552004-2552420 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT34368 NZ_AP022069:2552004-2552420 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACAGTTGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCGTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGACGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAATTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A0D8WF76