Detailed information of TA system TA034333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4214321..4215141
• Replicon: chromosome
• Accession: NZ_AP022064
• Organism: Escherichia coli strain WP4-W18-ESBL-08

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: H7R03_RS20420
• Protein ID: WP_001054379.1
• Coordinates: 4214321..4214578 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: H7R03_RS20425
• Protein ID: WP_000123957.1
• Coordinates: 4214590..4215141 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R03_RS20390	4209362..4210063	+	702	WP_000633597.1	pirin family protein	-
H7R03_RS20395	4210086..4210250	+	165	WP_001295544.1	hypothetical protein	-
H7R03_RS20400	4210311..4211621	-	1311	WP_000460528.1	serine dehydratase subunit alpha family protein	-
H7R03_RS20405	4211649..4212527	-	879	Protein_4006	septum formation protein	-
H7R03_RS20410	4212600..4213747	+	1148	WP_085949154.1	IS3-like element ISEc52 family transposase	-
H7R03_RS20415	4213780..4213944	+	165	Protein_4008	GNAT family N-acetyltransferase	-
H7R03_RS20420	4214321..4214578	+	258	WP_001054379.1	YjhX family toxin	Toxin
H7R03_RS20425	4214590..4215141	+	552	WP_000123957.1	N-acetyltransferase	Antitoxin
H7R03_RS20430	4215193..4215939	+	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
H7R03_RS20435	4216067..4216327	+	261	WP_000077645.1	hypothetical protein	-
H7R03_RS25275	4216365..4216481	+	117	Protein_4013	VOC family protein	-
H7R03_RS20440	4216726..4217847	+	1122	WP_000010833.1	M42 family metallopeptidase	-
H7R03_RS20445	4217844..4218122	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
H7R03_RS20450	4218134..4219447	+	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T34333 WP_001054379.1 NZ_AP022064:4214321-4214578 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T34333 NZ_AP022064:4214321-4214578 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT34333 WP_000123957.1 NZ_AP022064:4214590-4215141 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT34333 NZ_AP022064:4214590-4215141 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030