Detailed information of TA system TA034308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 290562..291362
• Replicon: chromosome
• Accession: NZ_AP022064
• Organism: Escherichia coli strain WP4-W18-ESBL-08

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: F4T503
• Locus tag: H7R03_RS01330
• Protein ID: WP_000342452.1
• Coordinates: 290835..291362 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: F4T504
• Locus tag: H7R03_RS01325
• Protein ID: WP_001277107.1
• Coordinates: 290562..290828 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R03_RS01305	286221..286889	+	669	WP_000617723.1	cell division ATP-binding protein FtsE	-
H7R03_RS01310	286882..287940	+	1059	WP_001042013.1	permease-like cell division protein FtsX	-
H7R03_RS01315	288185..289039	+	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
H7R03_RS01320	289310..290413	+	1104	WP_001021994.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
H7R03_RS01325	290562..290828	+	267	WP_001277107.1	DUF1778 domain-containing protein	Antitoxin
H7R03_RS01330	290835..291362	+	528	WP_000342452.1	GNAT family N-acetyltransferase	Toxin
H7R03_RS01335	291359..291742	-	384	WP_000778796.1	aspartate 1-decarboxylase autocleavage activator PanM	-
H7R03_RS01340	292165..293274	+	1110	WP_001296485.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
H7R03_RS01345	293322..294248	+	927	WP_001295097.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
H7R03_RS01350	294245..295522	+	1278	WP_000803819.1	branched chain amino acid ABC transporter permease LivM	-
H7R03_RS01355	295519..296286	+	768	WP_000082099.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19673.57 Da
• Isoelectric Point: 6.6348

>T34308 WP_000342452.1 NZ_AP022064:290835-291362 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYQSLGFIPLVGENENAL
FFPTKSIELLFTPDD

Nucleotide

• Length: 528 bp

>T34308 NZ_AP022064:290835-291362 [Escherichia coli]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCATT
AAATCTCTTTCTGACGACACATCTGGTTCGTCAACATCGCAATAAAATTCTGCGAGCGTATATCCTGTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATCCGGCAGTTGTTTTGAACGAGCCGCACTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTAACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAAAGTTGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATCAGTCGCTGGGCTTTATCCCATTGGTCGGAGAAAACGAAAATGCGTTA
TTTTTCCCGACCAAATCTATAGAGCTACTTTTTACACCAGACGATTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9929.38 Da
• Isoelectric Point: 10.0863

>AT34308 WP_001277107.1 NZ_AP022064:290562-290828 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRMILTEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT34308 NZ_AP022064:290562-290828 [Escherichia coli]
ATGTCTGCTGTTAAAAAGCAGCGTATTGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGAGCTGCGGAAGTGATTGAACAGCATCGGCGGA
TGATTCTCACTGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA

Structures

Toxin

• AlphaFold DB: A0A061K5K9

Antitoxin

• AlphaFold DB: A0A061YQ57