Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 106443..107086 Replicon plasmid pWP4-W18-ESBL-05_1
Accession NZ_AP022058
Organism Klebsiella sp. WP4-W18-ESBL-05

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag H7R58_RS24355 Protein ID WP_001044770.1
Coordinates 106670..107086 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag H7R58_RS24350 Protein ID WP_001261282.1
Coordinates 106443..106673 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R58_RS24315 101780..102652 - 873 Protein_113 hypothetical protein -
H7R58_RS24320 102884..103510 + 627 WP_008502208.1 ParA family plasmid-partitioning AAA ATPase -
H7R58_RS24325 103507..103812 + 306 WP_035894350.1 hypothetical protein -
H7R58_RS24330 104010..104792 - 783 WP_035894351.1 site-specific integrase -
H7R58_RS24335 104859..105467 - 609 WP_035894352.1 hypothetical protein -
H7R58_RS24340 105512..105868 - 357 WP_035894354.1 hypothetical protein -
H7R58_RS24345 106026..106486 - 461 Protein_119 hypothetical protein -
H7R58_RS24350 106443..106673 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H7R58_RS24355 106670..107086 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
H7R58_RS24360 107160..108722 + 1563 WP_182934823.1 AAA family ATPase -
H7R58_RS24365 108707..109729 + 1023 WP_000361404.1 helicase UvrD -
H7R58_RS24370 110267..111181 + 915 WP_169557270.1 HNH endonuclease -
H7R58_RS24375 111367..111717 - 351 WP_004187110.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..171435 171435


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T34307 WP_001044770.1 NZ_AP022058:106670-107086 [Klebsiella sp. WP4-W18-ESBL-05]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T34307 NZ_AP022058:106670-107086 [Klebsiella sp. WP4-W18-ESBL-05]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT34307 WP_001261282.1 NZ_AP022058:106443-106673 [Klebsiella sp. WP4-W18-ESBL-05]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT34307 NZ_AP022058:106443-106673 [Klebsiella sp. WP4-W18-ESBL-05]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References