Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 61573..62309 Replicon plasmid pWP4-W18-ESBL-05_1
Accession NZ_AP022058
Organism Klebsiella sp. WP4-W18-ESBL-05

Toxin (Protein)


Gene name tacT Uniprot ID A0A7I6S3M8
Locus tag H7R58_RS24110 Protein ID WP_035896699.1
Coordinates 61827..62309 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A7I6S5M8
Locus tag H7R58_RS24105 Protein ID WP_035896700.1
Coordinates 61573..61839 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R58_RS24090 58069..58983 + 915 WP_035896705.1 4-hydroxybenzoate octaprenyltransferase -
H7R58_RS24095 59899..60596 - 698 Protein_69 IS1 family transposase -
H7R58_RS24100 60852..61040 - 189 Protein_70 MFS transporter -
H7R58_RS24105 61573..61839 + 267 WP_035896700.1 DUF1778 domain-containing protein Antitoxin
H7R58_RS24110 61827..62309 + 483 WP_035896699.1 GNAT family N-acetyltransferase Toxin
H7R58_RS24115 62467..63668 - 1202 Protein_73 IS3 family transposase -
H7R58_RS24120 63809..64771 - 963 WP_051910814.1 M48 family metalloprotease -
H7R58_RS24125 64758..65507 - 750 WP_081918585.1 phosphate-starvation-inducible PsiE family protein -
H7R58_RS24130 65745..65942 - 198 WP_035896695.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..171435 171435
- inside IScluster/Tn - - 59899..63494 3595


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(46-149)

Antitoxin

Vibrio phage ICP1, Orf50

(2-87)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17280.93 Da        Isoelectric Point: 8.7197

>T34306 WP_035896699.1 NZ_AP022058:61827-62309 [Klebsiella sp. WP4-W18-ESBL-05]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAYHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T34306 NZ_AP022058:61827-62309 [Klebsiella sp. WP4-W18-ESBL-05]
GTGGGATGTATAACTGCGCCCGAGCCGTTATCCAGCGCCCATCAACTGGCAGAGTTCGTCAGTGGGGAAACGGTACTCGA
TGAATGGTTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCACGAACTTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTTTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTTCACGATGCGGTTCTACGCTGTTATCGCGTAGCTGAGAATATTGGCGTTCGTGCGATAATGGTTCACGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTTACCATGGATTTAAGGCATCGCAAACTCATGAGCGGACTCTGTTCCTTAAACTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10173.63 Da        Isoelectric Point: 4.5256

>AT34306 WP_035896700.1 NZ_AP022058:61573-61839 [Klebsiella sp. WP4-W18-ESBL-05]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPPINKLL
ARKPQWDV

Download         Length: 267 bp

>AT34306 NZ_AP022058:61573-61839 [Klebsiella sp. WP4-W18-ESBL-05]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGGGCACTCATCGATGCCGCTGCAGAAATCCTTCA
TAAATCGCGAACCGACTTCATTCTTGAGATGGCCTGCAAGGCTGCGGAGAACGTGATCCTTGATCGTCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCTCCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGATGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7I6S3M8

Antitoxin

Source ID Structure
AlphaFold DB A0A7I6S5M8

References