Detailed information of TA system TA034304

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 4343978..4344554
• Replicon: chromosome
• Accession: NZ_AP022057
• Organism: Klebsiella sp. WP4-W18-ESBL-05

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: H7R58_RS20805
• Protein ID: WP_182929534.1
• Coordinates: 4344267..4344554 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: H7R58_RS20800
• Protein ID: WP_106930461.1
• Coordinates: 4343978..4344280 (-)
• Length: 101 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R58_RS20780	4340705..4341448	+	744	WP_182929530.1	AraC family transcriptional regulator	-
H7R58_RS20785	4341496..4342137	+	642	WP_182929531.1	LysE family translocator	-
H7R58_RS20790	4342178..4342342	-	165	WP_182929532.1	DUF1127 domain-containing protein	-
H7R58_RS20795	4342521..4343933	+	1413	WP_182929533.1	PLP-dependent aminotransferase family protein	-
H7R58_RS20800	4343978..4344280	-	303	WP_106930461.1	BrnA antitoxin family protein	Antitoxin
H7R58_RS20805	4344267..4344554	-	288	WP_182929534.1	BrnT family toxin	Toxin
H7R58_RS20810	4344636..4345616	+	981	WP_000019445.1	IS5-like element ISKpn26 family transposase	-
H7R58_RS20815	4345888..4346130	+	243	WP_182929539.1	DUF2164 family protein	-
H7R58_RS20820	4346127..4346597	+	471	WP_182929540.1	GNAT family N-acetyltransferase	-
H7R58_RS20825	4346684..4347760	-	1077	WP_182929541.1	glycoside hydrolase family 88 protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	4344636..4345616	980

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (37-93)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11205.57 Da
• Isoelectric Point: 6.9900

>T34304 WP_182929534.1 NZ_AP022057:c4344554-4344267 [Klebsiella sp. WP4-W18-ESBL-05]
MPMEFEWDANKAQNNLRKHGVRFEDAVLVFDDPQHLSRQDRYENGEYRWQTIGLVHGIVVILVAHSVQFESGHEVIRIIS
ARKADRKERSRYEQG

Nucleotide

• Length: 288 bp

>T34304 NZ_AP022057:c4344554-4344267 [Klebsiella sp. WP4-W18-ESBL-05]
ATGCCAATGGAGTTTGAGTGGGATGCAAACAAGGCACAAAATAACCTGAGGAAACACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTATTTGACGATCCCCAACATCTGTCGCGGCAGGATCGATATGAGAATGGCGAGTATCGCTGGCAGACCATCGGTC
TTGTTCATGGCATTGTGGTTATTCTGGTTGCCCACAGCGTTCAGTTCGAAAGTGGTCATGAAGTGATTCGCATCATCAGC
GCCAGAAAGGCAGACCGTAAAGAGAGGAGTCGTTATGAACAGGGTTAA

Antitoxin

Protein

• Length: 101 a.a.
• Molecular weight: 11570.06 Da
• Isoelectric Point: 9.9257

>AT34304 WP_106930461.1 NZ_AP022057:c4344280-4343978 [Klebsiella sp. WP4-W18-ESBL-05]
MNRVKHKRGSVSTLSPQHEAELKALANKSDDEIDYSDIPATEDEQWSEANRGKFFRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLREQNKK

Nucleotide

• Length: 303 bp

>AT34304 NZ_AP022057:c4344280-4343978 [Klebsiella sp. WP4-W18-ESBL-05]
ATGAACAGGGTTAAGCATAAACGCGGCAGCGTATCCACGCTGAGTCCTCAGCATGAGGCTGAACTGAAAGCGCTGGCGAA
TAAATCGGATGATGAAATTGATTACAGCGACATTCCTGCGACAGAAGATGAGCAGTGGTCAGAGGCTAATCGCGGTAAGT
TTTTCCGTCCGTTGAAAACGCAGGCGTCTGTGCGTATTGATGCTGACGTGATGGAATGGTTGAAACGCCCTGGAAAGGGT
TACCAGACGCGGCTCAACGCCATTTTGCGCGAAGCGATGCTGCGCGAGCAAAATAAGAAATAA