Detailed information of TA system TA034291

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yafW-ykfI/CbtA-CbeA
• Location: 1026157..1026836
• Replicon: chromosome
• Accession: NZ_AP022057
• Organism: Klebsiella sp. WP4-W18-ESBL-05

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: A0A2I8S2T2
• Locus tag: H7R58_RS04910
• Protein ID: WP_008324514.1
• Coordinates: 1026157..1026498 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yafW
• Uniprot ID: A0A2I8S2U6
• Locus tag: H7R58_RS04915
• Protein ID: WP_008324516.1
• Coordinates: 1026519..1026836 (-)
• Length: 106 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R58_RS04895	1022401..1023402	-	1002	WP_182928677.1	LacI family DNA-binding transcriptional regulator	-
H7R58_RS04900	1023713..1025050	+	1338	WP_106926115.1	PTS sugar transporter subunit IIC	-
H7R58_RS04905	1025058..1025747	+	690	Protein_963	glycoside hydrolase family 1 protein	-
H7R58_RS04910	1026157..1026498	-	342	WP_008324514.1	type IV toxin-antitoxin system toxin YkfI	Toxin
H7R58_RS04915	1026519..1026836	-	318	WP_008324516.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H7R58_RS04920	1026849..1027076	-	228	WP_008324517.1	DUF987 domain-containing protein	-
H7R58_RS04925	1027085..1027561	-	477	WP_000811693.1	RadC family protein	-
H7R58_RS04930	1027577..1028035	-	459	WP_000211838.1	antirestriction protein	-
H7R58_RS04935	1028133..1028372	-	240	WP_000194654.1	DUF905 domain-containing protein	-
H7R58_RS04940	1028449..1028916	-	468	WP_001547765.1	hypothetical protein	-
H7R58_RS04945	1028939..1029133	-	195	Protein_971	hypothetical protein	-
H7R58_RS04950	1029188..1029892	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
H7R58_RS04955	1030072..1031754	+	1683	WP_103283513.1	MCP four helix bundle domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (6-104)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12919.98 Da
• Isoelectric Point: 9.6543

>T34291 WP_008324514.1 NZ_AP022057:c1026498-1026157 [Klebsiella sp. WP4-W18-ESBL-05]
MKTLPATTQRAAKPCLSPVAVWQMLLTRLLEQHYGLTINDTPFCNEAVIKEHIDAGITLADAVNFLVEKYELVRIDRKGF
NWQEQSPYLRAVDILRARQATGLLRQSRNNVVR

Nucleotide

• Length: 342 bp

>T34291 NZ_AP022057:c1026498-1026157 [Klebsiella sp. WP4-W18-ESBL-05]
ATGAAAACTTTACCTGCAACAACTCAGCGGGCGGCGAAGCCCTGCCTGTCACCCGTGGCTGTCTGGCAAATGTTACTGAC
ACGTCTGCTGGAACAGCACTATGGTCTGACAATAAACGACACGCCATTCTGCAATGAGGCTGTGATTAAGGAACACATCG
ATGCCGGTATCACCCTAGCCGATGCCGTGAATTTTCTGGTGGAAAAATACGAACTGGTGCGTATCGACAGGAAGGGATTT
AACTGGCAGGAACAATCCCCTTATCTCCGGGCTGTAGACATTCTGCGAGCACGGCAGGCAACTGGCTTGTTGCGGCAAAG
CCGTAACAACGTAGTACGATGA

Antitoxin

Protein

• Length: 106 a.a.
• Molecular weight: 11787.33 Da
• Isoelectric Point: 7.3476

>AT34291 WP_008324516.1 NZ_AP022057:c1026836-1026519 [Klebsiella sp. WP4-W18-ESBL-05]
MSNPTRGLQRAITPRLGARLVQEGNRLHYLADRASITGRFSDTECRKLDETCPHFIRHMESMLTTGELSPQHAHCVTLYH
NGFTCEADTLGSCGYVYIAIYPTQS

Nucleotide

• Length: 318 bp

>AT34291 NZ_AP022057:c1026836-1026519 [Klebsiella sp. WP4-W18-ESBL-05]
ATGAGCAACCCTACCAGGGGCCTGCAGCGGGCTATCACGCCGCGCCTGGGAGCCCGTCTGGTGCAGGAGGGTAACCGACT
GCACTATCTGGCTGACAGGGCCAGCATCACCGGCAGGTTCAGTGACACCGAATGCCGGAAGCTGGATGAAACATGCCCGC
ACTTTATCCGCCATATGGAGTCGATGCTAACCACCGGTGAACTCAGTCCCCAACACGCCCACTGCGTCACCCTGTACCAC
AACGGTTTCACCTGCGAAGCCGATACTCTTGGCAGTTGCGGCTACGTATACATCGCCATTTACCCCACTCAGAGTTAA

Structures

Toxin

• AlphaFold DB: A0A2I8S2T2

Antitoxin

• AlphaFold DB: A0A2I8S2U6