Detailed information of TA system TA034287

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 370364..370950
• Replicon: chromosome
• Accession: NZ_AP022057
• Organism: Klebsiella sp. WP4-W18-ESBL-05

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: H7R58_RS01755
• Protein ID: WP_106925089.1
• Coordinates: 370582..370950 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: H7R58_RS01750
• Protein ID: WP_181357971.1
• Coordinates: 370364..370585 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R58_RS01725	365419..366528	+	1110	WP_106925084.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
H7R58_RS01730	366583..367509	+	927	WP_106925085.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
H7R58_RS01735	367506..368786	+	1281	WP_106925086.1	branched chain amino acid ABC transporter permease LivM	-
H7R58_RS01740	368783..369550	+	768	WP_182929886.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
H7R58_RS01745	369552..370265	+	714	WP_106925088.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
H7R58_RS01750	370364..370585	+	222	WP_181357971.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H7R58_RS01755	370582..370950	+	369	WP_106925089.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
H7R58_RS01760	371183..372499	+	1317	WP_182929887.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
H7R58_RS01765	372600..373487	+	888	WP_106925091.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
H7R58_RS01770	373484..374329	+	846	WP_106925092.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
H7R58_RS01775	374332..375402	+	1071	WP_182929888.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	367506..376139	8633

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (6-119)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13526.80 Da
• Isoelectric Point: 6.7249

>T34287 WP_106925089.1 NZ_AP022057:370582-370950 [Klebsiella sp. WP4-W18-ESBL-05]
MTLQIISAEEIIQFHDRLLRVTPGVEGMPDPGRAEALMYRVLNQIEYQGVTDVWLLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLPANPDFVAMTVEAAAGQLTLEQIAARLRG

Nucleotide

• Length: 369 bp

>T34287 NZ_AP022057:370582-370950 [Klebsiella sp. WP4-W18-ESBL-05]
ATGACCCTTCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGTTGCTCCGCGTAACGCCTGGCGTAGAGGG
AATGCCCGATCCCGGCCGCGCAGAAGCGCTGATGTACCGGGTGCTCAATCAAATCGAATATCAAGGCGTGACAGATGTCT
GGCTGCTTGCGGCAATGCATCTGCTGGCGATCTCGCGTGGGCATATTTTTAATGACGGAAATAAACGGACGGCATTATTT
ATTACCTTACTGTTTTTAAAGCGTAACGGCATTTCATTACCGGCTAATCCTGATTTTGTTGCGATGACGGTTGAGGCTGC
CGCTGGGCAACTCACGCTGGAGCAGATAGCAGCCCGGCTCCGTGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8146.18 Da
• Isoelectric Point: 5.0775

>AT34287 WP_181357971.1 NZ_AP022057:370364-370585 [Klebsiella sp. WP4-W18-ESBL-05]
MRTINYSEARQNLAEVLESAVSGGPVTITRRGHKPAVIISAEEFERYQAARMDDEFAAIMAIHGNEIRELADK

Nucleotide

• Length: 222 bp

>AT34287 NZ_AP022057:370364-370585 [Klebsiella sp. WP4-W18-ESBL-05]
ATGCGTACGATTAACTACAGTGAAGCGCGGCAGAATCTGGCGGAGGTACTGGAAAGTGCCGTCAGCGGGGGGCCTGTCAC
CATTACGCGCCGAGGGCATAAGCCTGCGGTGATTATCAGCGCGGAAGAATTTGAGCGTTATCAGGCGGCCAGAATGGATG
ATGAATTCGCGGCAATCATGGCGATACATGGTAATGAAATCAGGGAGCTGGCAGATAAATGA