Detailed information of TA system TA034272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2615122..2615760
• Replicon: chromosome
• Accession: NZ_AP022049
• Organism: Escherichia coli strain WP4-S17-ESBL-08

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: H7R14_RS12345
• Protein ID: WP_000813795.1
• Coordinates: 2615584..2615760 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: H7R14_RS12340
• Protein ID: WP_076797675.1
• Coordinates: 2615122..2615538 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R14_RS12320	2610274..2611215	-	942	WP_001251315.1	ABC transporter permease	-
H7R14_RS12325	2611216..2612229	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
H7R14_RS12330	2612247..2613392	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
H7R14_RS12335	2613637..2615043	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
H7R14_RS12340	2615122..2615538	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
H7R14_RS12345	2615584..2615760	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
H7R14_RS12350	2615982..2616212	+	231	WP_023910283.1	YncJ family protein	-
H7R14_RS12355	2616304..2618265	-	1962	WP_182915530.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
H7R14_RS12360	2618338..2618874	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
H7R14_RS12365	2618927..2620138	+	1212	WP_071591517.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T34272 WP_000813795.1 NZ_AP022049:c2615760-2615584 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T34272 NZ_AP022049:c2615760-2615584 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTAAAACTCAG
GTTTCACGGGAGGCGCAGTGTCATGCCGCGTCACCCCAGCGAAGAGATTAAAGAACCATTGCGTAAAGCCATTCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT34272 WP_076797675.1 NZ_AP022049:c2615538-2615122 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT34272 NZ_AP022049:c2615538-2615122 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATCAAATAGTCACGATCACTTTATTGAAGTGCCTTTGAGCGTCGCCTCTAAAGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAAAT
TACTCGCTTATTTAACTTGCACCATGCGACAAAAATCGACGCCGTCCAGCTTGCGGCTAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1