Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 127006..127607 Replicon plasmid pWP4-S17-ESBL-03_1
Accession NZ_AP022045
Organism Escherichia coli strain WP4-S17-ESBL-03

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag H7R12_RS25290 Protein ID WP_001216034.1
Coordinates 127006..127386 (-) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag H7R12_RS25295 Protein ID WP_001190712.1
Coordinates 127386..127607 (-) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R12_RS25255 122057..122989 - 933 WP_000081352.1 homocysteine S-methyltransferase -
H7R12_RS25260 122976..124379 - 1404 WP_001373486.1 S-methylmethionine permease -
H7R12_RS25265 124587..125603 + 1017 WP_012372828.1 IS5-like element IS5 family transposase -
H7R12_RS25270 125831..126148 + 318 WP_001513659.1 hypothetical protein -
H7R12_RS25275 126098..126511 - 414 Protein_153 DDE-type integrase/transposase/recombinase -
H7R12_RS25280 126435..126794 - 360 WP_001513660.1 hypothetical protein -
H7R12_RS25285 126822..127001 - 180 WP_001513661.1 hypothetical protein -
H7R12_RS25290 127006..127386 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
H7R12_RS25295 127386..127607 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
H7R12_RS25300 127790..129346 + 1557 WP_001617892.1 type I restriction-modification system subunit M -
H7R12_RS25305 129343..130092 + 750 Protein_159 restriction endonuclease subunit S -
H7R12_RS25310 130434..131131 + 698 Protein_160 IS1 family transposase -
H7R12_RS25315 131268..132200 - 933 WP_000991832.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaCTX-M-27 / tet(A) senB 1..148598 148598
- inside IScluster/Tn - - 121100..131131 10031


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T34254 WP_001216034.1 NZ_AP022045:c127386-127006 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T34254 NZ_AP022045:c127386-127006 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT34254 WP_001190712.1 NZ_AP022045:c127607-127386 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT34254 NZ_AP022045:c127607-127386 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References