Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4094316..4094836 Replicon chromosome
Accession NZ_AP022044
Organism Escherichia coli strain WP4-S17-ESBL-03

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag H7R12_RS19985 Protein ID WP_001160964.1
Coordinates 4094316..4094600 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag H7R12_RS19990 Protein ID WP_000125566.1
Coordinates 4094603..4094836 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R12_RS19960 4089961..4091247 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
H7R12_RS19965 4091247..4092236 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
H7R12_RS19970 4092233..4093054 + 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
H7R12_RS19975 4093057..4093434 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
H7R12_RS19980 4093441..4094289 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
H7R12_RS19985 4094316..4094600 - 285 WP_001160964.1 CcdB family protein Toxin
H7R12_RS19990 4094603..4094836 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
H7R12_RS19995 4094922..4095401 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
H7R12_RS20000 4095593..4097455 - 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
H7R12_RS20005 4097448..4097978 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
H7R12_RS20010 4098086..4099417 - 1332 WP_001183209.1 MFS transporter -
H7R12_RS20015 4099476..4099763 - 288 WP_000203744.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T34248 WP_001160964.1 NZ_AP022044:c4094600-4094316 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T34248 NZ_AP022044:c4094600-4094316 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT34248 WP_000125566.1 NZ_AP022044:c4094836-4094603 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT34248 NZ_AP022044:c4094836-4094603 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References