Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3641850..3642468 Replicon chromosome
Accession NZ_AP022044
Organism Escherichia coli strain WP4-S17-ESBL-03

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H7R12_RS17830 Protein ID WP_001291435.1
Coordinates 3642250..3642468 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H7R12_RS17825 Protein ID WP_000344800.1
Coordinates 3641850..3642224 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R12_RS17815 3636939..3638132 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H7R12_RS17820 3638155..3641304 + 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
H7R12_RS17825 3641850..3642224 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H7R12_RS17830 3642250..3642468 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
H7R12_RS17835 3642642..3643193 + 552 WP_000102539.1 maltose O-acetyltransferase -
H7R12_RS17840 3643309..3643779 + 471 WP_000136192.1 YlaC family protein -
H7R12_RS17845 3643943..3645493 + 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H7R12_RS17850 3645535..3645888 - 354 WP_000878135.1 DUF1428 family protein -
H7R12_RS17860 3646267..3646578 + 312 WP_000409908.1 MGMT family protein -
H7R12_RS17865 3646609..3647181 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T34246 WP_001291435.1 NZ_AP022044:3642250-3642468 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T34246 NZ_AP022044:3642250-3642468 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT34246 WP_000344800.1 NZ_AP022044:3641850-3642224 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT34246 NZ_AP022044:3641850-3642224 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References