Detailed information of TA system TA034225

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 2428783..2429476
• Replicon: chromosome
• Accession: NZ_AP022038
• Organism: Aeromonas veronii strain WP3-W19-ESBL-03

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: H7R62_RS11245
• Protein ID: WP_000182276.1
• Coordinates: 2429120..2429476 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: N2IIN5
• Locus tag: H7R62_RS11240
• Protein ID: WP_001172026.1
• Coordinates: 2428783..2429118 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R62_RS11200	2423788..2424060	+	273	WP_182939745.1	hypothetical protein	-
H7R62_RS11205	2424273..2425703	-	1431	WP_182939680.1	16S rRNA (cytosine(1407)-C(5))-methyltransferase RsmF	-
H7R62_RS11210	2425781..2426176	-	396	Protein_2166	recombinase family protein	-
H7R62_RS11215	2426343..2427257	-	915	WP_001736514.1	HD domain-containing protein	-
H7R62_RS11220	2427304..2427588	-	285	WP_130627582.1	DUF3391 domain-containing protein	-
H7R62_RS11225	2427706..2428086	-	381	WP_001054412.1	hypothetical protein	-
H7R62_RS11230	2428083..2428409	-	327	WP_000091614.1	hypothetical protein	-
H7R62_RS11235	2428433..2428768	-	336	WP_000741275.1	hypothetical protein	-
H7R62_RS11240	2428783..2429118	-	336	WP_001172026.1	helix-turn-helix domain-containing protein	Antitoxin
H7R62_RS11245	2429120..2429476	-	357	WP_000182276.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R62_RS11250	2429668..2430270	+	603	WP_010465829.1	recombinase family protein	-
H7R62_RS11255	2430254..2433283	+	3030	WP_032432545.1	Tn3 family transposase	-
H7R62_RS11260	2433240..2434373	-	1134	WP_197970800.1	replication protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	aac(6')-Iag / ant(3'')-Ia / sul1 / blaCMY-8	-	2425781..2477075	51294

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (16-109)

Antitoxin

• Cro/C1-type helix-turn-helix domain (40-93)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12912.93 Da
• Isoelectric Point: 8.9018

>T34225 WP_000182276.1 NZ_AP022038:c2429476-2429120 [Aeromonas veronii]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKELKGFGGAGVLKVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQEL

Nucleotide

• Length: 357 bp

>T34225 NZ_AP022038:c2429476-2429120 [Aeromonas veronii]
ATGACAAACAAAGAAAAACCGCTCGAATGGATCGCGAGCAGCCACAAGGATTTGATGGCGTTGCCGTCCGACGTGCGTCG
CCGTTTCGGTTACGCGCTCTCGTTGGCGCAGATAGGCGATCAGGATGACGCAGCAAAGGAGCTCAAGGGGTTCGGTGGTG
CCGGCGTGCTGAAGGTCGTCGAAGACGATGCCGGCGGCACCTATCGAGCGGTATACACGGTCAAGTTTGCGGAAGCGGTG
TTCGTCCTGCACTGCTTCCAGAAGAAGAGCAAGAGCGGAATCGCCACGCCAAAGGCCGACATGGACATCATCCGCGCTCG
GCTGAAGGTGGCCGAGGTATTGGCACAGGAGCTATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12201.39 Da
• Isoelectric Point: 10.6058

>AT34225 WP_001172026.1 NZ_AP022038:c2429118-2428783 [Aeromonas veronii]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Nucleotide

• Length: 336 bp

>AT34225 NZ_AP022038:c2429118-2428783 [Aeromonas veronii]
ATGCAAAAACGAATCATTGAAGGCGTCGAGGTTCAGCGCAGCTCGGGCAACGTCTTTGCCGACCTTGGACTGCCTGACGC
TGAAAAGCTCAAGATCAAGACCGGCCTGGTGGTCGAGATCAGGAGGGCCATGCGCGCCCTTGGGCTGACTCAACAAGCGG
CGGCCAAACGCATGGGCATCCCGCAACCGAAGGTGTCGGGCATGATGCGCGGCGACTTCACCAATCTATCCGAACGCAAG
CTGATGGATTGTCTGAATCGCCTCGGCTACGACATCGAAATCAAGGTACGGCCAGCAGCCGAGCCGATCGGGCATCTAAC
GCTCGCAACCGCTTAA

Structures

Antitoxin

• AlphaFold DB: A0A024EKI7