Detailed information of TA system TA034216

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4008144..4008964
• Replicon: chromosome
• Accession: NZ_AP022036
• Organism: Escherichia coli strain WP3-S18-ESBL-09

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: H7R01_RS19180
• Protein ID: WP_001054376.1
• Coordinates: 4008144..4008401 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: H7R01_RS19185
• Protein ID: WP_000215866.1
• Coordinates: 4008413..4008964 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R01_RS19160	4003430..4004536	+	1107	WP_001044124.1	N-acetylneuraminate epimerase	-
H7R01_RS19165	4004601..4005581	+	981	WP_001765419.1	sialate O-acetylesterase	-
H7R01_RS19170	4006164..4007390	-	1227	Protein_3746	DNA helicase	-
H7R01_RS19175	4007468..4007767	+	300	WP_024186563.1	GNAT family N-acetyltransferase	-
H7R01_RS19180	4008144..4008401	+	258	WP_001054376.1	hypothetical protein	Toxin
H7R01_RS19185	4008413..4008964	+	552	WP_000215866.1	N-acetyltransferase	Antitoxin
H7R01_RS19190	4009016..4009762	+	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
H7R01_RS19195	4009889..4010149	+	261	WP_000084876.1	hypothetical protein	-
H7R01_RS23255	4010187..4010303	+	117	Protein_3752	VOC family protein	-
H7R01_RS19200	4010548..4011669	+	1122	WP_000010835.1	M42 family metallopeptidase	-
H7R01_RS19205	4011666..4011944	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
H7R01_RS19210	4011956..4013269	+	1314	WP_000460846.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimC / fimI / fimA / fimE / fimB	3997172..4017184	20012

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T34216 WP_001054376.1 NZ_AP022036:4008144-4008401 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T34216 NZ_AP022036:4008144-4008401 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20493.64 Da
• Isoelectric Point: 6.8758

>AT34216 WP_000215866.1 NZ_AP022036:4008413-4008964 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKETDLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT34216 NZ_AP022036:4008413-4008964 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAACCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCGACCTTCAAAGGAGAAATGGATTCACCGCTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: S1PA82