Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 3961247..3961823 Replicon chromosome
Accession NZ_AP022036
Organism Escherichia coli strain WP3-S18-ESBL-09

Toxin (Protein)


Gene name shpA Uniprot ID A0A2U2VE01
Locus tag H7R01_RS18970 Protein ID WP_001131965.1
Coordinates 3961536..3961823 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A2S5UGC7
Locus tag H7R01_RS18965 Protein ID WP_019842999.1
Coordinates 3961247..3961549 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R01_RS18945 3956941..3957453 + 513 WP_001175455.1 4-hydroxyphenylacetate 3-monooxygenase reductase subunit -
H7R01_RS18950 3957843..3959993 + 2151 WP_001439331.1 pyruvate/proton symporter BtsT -
H7R01_RS18955 3960043..3960246 + 204 WP_000467859.1 YbdD/YjiX family protein -
H7R01_RS18960 3960257..3961213 + 957 WP_001295525.1 GTPase -
H7R01_RS18965 3961247..3961549 - 303 WP_019842999.1 BrnA antitoxin family protein Antitoxin
H7R01_RS18970 3961536..3961823 - 288 WP_001131965.1 BrnT family toxin Toxin
H7R01_RS18975 3962020..3963186 + 1167 WP_000800825.1 restriction endonuclease -
H7R01_RS18980 3963250..3964869 + 1620 WP_001029743.1 type I restriction-modification system subunit M -
H7R01_RS18985 3964859..3966283 + 1425 WP_000535015.1 restriction endonuclease subunit S -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11223.71 Da        Isoelectric Point: 7.4021

>T34215 WP_001131965.1 NZ_AP022036:c3961823-3961536 [Escherichia coli]
MPMEFEWDANKAKSNRVKHGIRFEDAVLVFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T34215 NZ_AP022036:c3961823-3961536 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTAAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11521.00 Da        Isoelectric Point: 9.8223

>AT34215 WP_019842999.1 NZ_AP022036:c3961549-3961247 [Escherichia coli]
MSMVKHKRGTSSTLNVQHEAELKALANKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT34215 NZ_AP022036:c3961549-3961247 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCAATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2U2VE01

Antitoxin

Source ID Structure
AlphaFold DB A0A2S5UGC7

References