Detailed information of TA system TA034200

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1374261..1374886
• Replicon: chromosome
• Accession: NZ_AP022036
• Organism: Escherichia coli strain WP3-S18-ESBL-09

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: H7R01_RS06725
• Protein ID: WP_000911330.1
• Coordinates: 1374488..1374886 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: U9XQV3
• Locus tag: H7R01_RS06720
• Protein ID: WP_000450524.1
• Coordinates: 1374261..1374488 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R01_RS06695	1370064..1370534	-	471	WP_001068682.1	thioredoxin-dependent thiol peroxidase	-
H7R01_RS06700	1370534..1371106	-	573	WP_000176187.1	glycine cleavage system transcriptional repressor	-
H7R01_RS06705	1371252..1372130	+	879	WP_001295469.1	4-hydroxy-tetrahydrodipicolinate synthase	-
H7R01_RS06710	1372147..1373181	+	1035	WP_001297320.1	outer membrane protein assembly factor BamC	-
H7R01_RS06715	1373394..1374107	+	714	WP_001295467.1	phosphoribosylaminoimidazolesuccinocarboxamide synthase	-
H7R01_RS06720	1374261..1374488	+	228	WP_000450524.1	toxin-antitoxin system antitoxin VapB	Antitoxin
H7R01_RS06725	1374488..1374886	+	399	WP_000911330.1	tRNA(fMet)-specific endonuclease VapC	Toxin
H7R01_RS06730	1375033..1375896	+	864	WP_001267507.1	neutral zinc metallopeptidase	-
H7R01_RS06735	1375911..1377926	+	2016	WP_000829332.1	tRNA cytosine(34) acetyltransferase TmcA	-
H7R01_RS06740	1378000..1378698	+	699	WP_000679812.1	esterase	-
H7R01_RS06745	1378808..1379008	-	201	WP_000383836.1	YpfN family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-52)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 14894.25 Da
• Isoelectric Point: 9.2216

>T34200 WP_000911330.1 NZ_AP022036:1374488-1374886 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLVVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Nucleotide

• Length: 399 bp

>T34200 NZ_AP022036:1374488-1374886 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8498.61 Da
• Isoelectric Point: 4.3867

>AT34200 WP_000450524.1 NZ_AP022036:1374261-1374488 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Nucleotide

• Length: 228 bp

>AT34200 NZ_AP022036:1374261-1374488 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Antitoxin

• AlphaFold DB: A0A829CM33