34191

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 36573..37098 Replicon plasmid pWP3-S18-ESBL-08_1
Accession NZ_AP022033
Organism Escherichia coli strain WP3-S18-ESBL-08

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag H7R15_RS24070 Protein ID WP_001159868.1
Coordinates 36793..37098 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag H7R15_RS24065 Protein ID WP_000813634.1
Coordinates 36573..36791 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R15_RS24030 31674..32033 - 360 WP_001513660.1 hypothetical protein -
H7R15_RS24035 32061..32240 - 180 WP_001513661.1 hypothetical protein -
H7R15_RS24040 32245..32625 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin -
H7R15_RS24045 32625..32846 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
H7R15_RS24050 33100..33797 + 698 Protein_37 IS1 family transposase -
H7R15_RS24055 33934..34866 - 933 WP_000991832.1 hypothetical protein -
H7R15_RS24060 34870..35865 - 996 WP_000246636.1 hypothetical protein -
H7R15_RS24065 36573..36791 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H7R15_RS24070 36793..37098 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
H7R15_RS24075 37099..37905 + 807 WP_000016982.1 site-specific integrase -
H7R15_RS24080 38679..39434 + 756 WP_000852146.1 replication initiation protein RepE -
H7R15_RS24085 40022..41188 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-27 senB 1..105867 105867
- inside IScluster/Tn - - 26339..33797 7458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T34191 WP_001159868.1 NZ_AP022033:36793-37098 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T34191 NZ_AP022033:36793-37098 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT34191 WP_000813634.1 NZ_AP022033:36573-36791 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT34191 NZ_AP022033:36573-36791 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
AlphaFold DB A0A829CQY2

References