Detailed information of TA system TA034185

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4142570..4143390
• Replicon: chromosome
• Accession: NZ_AP022032
• Organism: Escherichia coli strain WP3-S18-ESBL-08

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: B6I030
• Locus tag: H7R15_RS20045
• Protein ID: WP_001054379.1
• Coordinates: 4142570..4142827 (+)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: -
• Locus tag: H7R15_RS20050
• Protein ID: WP_000123957.1
• Coordinates: 4142839..4143390 (+)
• Length: 184 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R15_RS20025	4138117..4139097	+	981	WP_000991462.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
H7R15_RS20030	4139680..4140786	-	1107	Protein_3928	DNA helicase	-
H7R15_RS20035	4140849..4141996	+	1148	WP_085949154.1	IS3-like element ISEc52 family transposase	-
H7R15_RS20040	4142029..4142193	+	165	Protein_3930	GNAT family N-acetyltransferase	-
H7R15_RS20045	4142570..4142827	+	258	WP_001054379.1	YjhX family toxin	Toxin
H7R15_RS20050	4142839..4143390	+	552	WP_000123957.1	N-acetyltransferase	Antitoxin
H7R15_RS20055	4143442..4144188	+	747	WP_000354249.1	class I SAM-dependent methyltransferase	-
H7R15_RS20060	4144316..4144576	+	261	WP_000077645.1	hypothetical protein	-
H7R15_RS24530	4144614..4144730	+	117	Protein_3935	VOC family protein	-
H7R15_RS20065	4144975..4146096	+	1122	WP_000010833.1	M42 family metallopeptidase	-
H7R15_RS20070	4146093..4146371	+	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
H7R15_RS20075	4146383..4147696	+	1314	WP_000460845.1	galactitol-specific PTS transporter subunit IIC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fimE	4131191..4151611	20420

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (19-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9568.07 Da
• Isoelectric Point: 11.1381

>T34185 WP_001054379.1 NZ_AP022032:4142570-4142827 [Escherichia coli]
MNLSRQEQRTLHVLAKGGRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTGLNNVRA
QLDNR

Nucleotide

• Length: 258 bp

>T34185 NZ_AP022032:4142570-4142827 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACGTACGTTACACGTTCTCGCTAAAGGTGGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGTCAGCCCTATCGAATCAACACTACCGGGCTGAATAACGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 184 a.a.
• Molecular weight: 20358.34 Da
• Isoelectric Point: 6.4182

>AT34185 WP_000123957.1 NZ_AP022032:4142839-4143390 [Escherichia coli]
MTAHHFTFQITDESDASDIREVETRAFGFSKEADLTASLLEDESARPALSLLARHEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQALTA
QPMNVTGHIQCAQALMKPEHWRE

Nucleotide

• Length: 552 bp

>AT34185 NZ_AP022032:4142839-4143390 [Escherichia coli]
ATGACAGCACACCACTTCACTTTTCAAATCACAGATGAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAACGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCTGCGTTATCGCTGCTGGCCC
GACATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGGGAAATGGATTCACCGCTGATGCAT
ATCCTCGCTCCCCTGGCAGTCATACCGGAATACCAGGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAAGCACTCACCGCT
CAGCCCATGAACGTGACCGGCCACATCCAGTGCGCGCAGGCCCTGATGAAACCGGAGCACTGGCGTGAATAA

Structures

Toxin

• AlphaFold DB: B6I030