Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 3983136..3983656 Replicon chromosome
Accession NZ_AP022032
Organism Escherichia coli strain WP3-S18-ESBL-08

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag H7R15_RS19280 Protein ID WP_001160964.1
Coordinates 3983136..3983420 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag H7R15_RS19285 Protein ID WP_000125566.1
Coordinates 3983423..3983656 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R15_RS19255 3978781..3980067 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
H7R15_RS19260 3980067..3981056 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
H7R15_RS19265 3981053..3981874 + 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
H7R15_RS19270 3981877..3982254 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
H7R15_RS19275 3982261..3983109 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
H7R15_RS19280 3983136..3983420 - 285 WP_001160964.1 CcdB family protein Toxin
H7R15_RS19285 3983423..3983656 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
H7R15_RS19290 3983742..3984221 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
H7R15_RS19295 3984413..3986275 - 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
H7R15_RS19300 3986268..3986798 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
H7R15_RS19305 3986906..3988237 - 1332 WP_001183209.1 MFS transporter -
H7R15_RS19310 3988296..3988583 - 288 WP_000203744.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T34183 WP_001160964.1 NZ_AP022032:c3983420-3983136 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T34183 NZ_AP022032:c3983420-3983136 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT34183 WP_000125566.1 NZ_AP022032:c3983656-3983423 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT34183 NZ_AP022032:c3983656-3983423 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References