Detailed information of TA system TA034182

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3715533..3717222
• Replicon: chromosome
• Accession: NZ_AP022032
• Organism: Escherichia coli strain WP3-S18-ESBL-08

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A061KTE0
• Locus tag: H7R15_RS18000
• Protein ID: WP_000365807.1
• Coordinates: 3715533..3716453 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: H7R15_RS18005
• Protein ID: WP_001295795.1
• Coordinates: 3716440..3717222 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R15_RS17960	3711006..3711716	+	711	WP_001295798.1	fimbrial chaperone EcpE	-
H7R15_RS17965	3712023..3712352	+	330	WP_001295797.1	YkgJ family cysteine cluster protein	-
H7R15_RS17970	3712364..3712527	-	164	Protein_3527	tail fiber assembly protein	-
H7R15_RS17975	3712601..3713215	-	615	WP_001019923.1	YagU family protein	-
H7R15_RS17980	3713430..3713615	-	186	WP_000146240.1	hypothetical protein	-
H7R15_RS17985	3713664..3713822	+	159	Protein_3530	integrase	-
H7R15_RS17990	3714049..3714384	+	336	WP_000996167.1	Arm DNA-binding domain-containing protein	-
H7R15_RS17995	3714592..3715368	+	777	WP_000171155.1	site-specific integrase	-
H7R15_RS18000	3715533..3716453	-	921	WP_000365807.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
H7R15_RS18005	3716440..3717222	-	783	WP_001295795.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
H7R15_RS18010	3717506..3720604	+	3099	WP_000673070.1	type I restriction endonuclease subunit R	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	fdeC / ykgK/ecpR / yagZ/ecpA / yagY/ecpB / yagX/ecpC / yagW/ecpD / yagV/ecpE	3675317..3724285	48968

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-223)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34570.89 Da
• Isoelectric Point: 7.3239

>T34182 WP_000365807.1 NZ_AP022032:c3716453-3715533 [Escherichia coli]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLGRPGLTREIFDGFLCYLAGHPRPIAELLASNWDTDRIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHFTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAMLKDKNPAKYVAAVNKLERVLE

Nucleotide

• Length: 921 bp

>T34182 NZ_AP022032:c3716453-3715533 [Escherichia coli]
ATGGATCTGCGTGATAGCTATGCCAGGCAGGTTAAACTGTTGATGGCCGCTCTGCCGCATGTGGCGAAGGAATCCTGTTT
CGCGTTGAAAGGCGGCACAGCAATCAATTTATTTGTGCAGGATTTCCCCCGTCTATCGGTGGATATCGATCTGGCGTATA
AGAGCTTTGCGGATCGCGATACCGATCTGGCGGCGATCAACGACGCTTTGAAGAGAATTACTGCATCGCTGAACGGCAGA
CCGGGGATCACCGCGATTCGGCAGGAAAATAAAGCGGATGAAAAGCGCATCATTGTGAATACCGCTGATGCAAAGATTAA
GATCGAGGTTAGCCCGGTCTGGCGCGGGCTGTTGTTGCCTCCGGCAGAAATGCCTGTGTGCGAACGTGTGGAAATGGAGT
ATGGCTTCACCACCATGAGTGTCGTCTCGCTGGCGGATCTTTATGGCGGTAAAATATGTGCTGCGCTTGATCGTCAGCAT
CCGCGCGATCTGTTTGATGTGTTGAATATGCTGGGGAGACCGGGCCTGACGCGCGAAATTTTTGACGGTTTTCTTTGTTA
TCTGGCAGGCCATCCCCGTCCCATTGCCGAGTTGCTGGCTTCAAACTGGGATACAGATCGGATTGCCACGTTATACGCTC
AGGAATTTTCGGGGATGACTCAGCAGGAAACGTCGCTGGAATCGCTTCTGTCAGTAACGACTTTGCTGCCGCAGGCGTTG
AAGTCACATTTTACGGATCTGGATCGCGAGTTCTTGCTCAGTTTTAAGCAAAACAGCCCTGACTGGTCGCGGTATCGCTA
TCCTGAAATTCAGCATCTTCCGGCTATTCGCTGGAAGCAACGCAATCTGGCGATGCTTAAAGATAAAAATCCGGCGAAGT
ATGTGGCGGCGGTGAATAAGCTAGAGAGGGTGCTGGAGTAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29099.24 Da
• Isoelectric Point: 8.5975

>AT34182 WP_001295795.1 NZ_AP022032:c3717222-3716440 [Escherichia coli]
MPNYLNWLMQNTLPGQVLLQSWLTRNGVDRKLSYLYVQNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVSSFTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGGNLLSPRKLQALLRASHSIKTNRVMLYLARRNGHPYFQYLDQSGIETGTGKRQIIPGGWLEP
DYQITVPRTFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT34182 NZ_AP022032:c3717222-3716440 [Escherichia coli]
TTGCCAAACTATCTAAACTGGTTGATGCAAAACACGCTACCCGGTCAGGTGCTGCTCCAGTCATGGCTTACCCGCAATGG
CGTAGATCGTAAGCTTTCTTATCTCTATGTTCAGAATGGCTGGCTCAGGCGCATCGCTCACGGTGTGTACTGCCGGACGG
GCCGTGAGCCGGATTGGGTAGACGCGGTTTACTGCCTTCAAACACAGTGGGAAAAGCCTGTTCGGGTGGCGGGGCTAACC
AGCCTTGCGCTTCAGGGCCATGCGCACTATACCGAACCGGGTAAGCCGCAGGTCTGGCTCGCGCTTCCGGCTTACGTCAA
ACTGCCGGGATGGTTTGCTGCCTTCGAACAATCAGCAACATTTATCACGCTGTATACATCGGGCCTTACCGTTGATGTCT
CGTCATTTACTACGGAACTGAAAATTGGCGACCGGCAGTTAGCAGGCAGTGCACCGGAACTGGCCGCGTATGAGATCGCC
TGCGGCGTTCCTTCGTTGATCTCGTTCGAACATGCCGATGCTCTGTTTCAGGGGGGGAATCTTCTCAGCCCACGCAAATT
GCAGGCACTGCTTCGCGCCAGTCATTCGATTAAAACCAACCGGGTGATGCTTTACCTTGCCCGGCGTAATGGACACCCTT
ATTTTCAGTATCTTGATCAAAGCGGTATAGAAACAGGTACGGGAAAGCGGCAAATCATACCTGGTGGCTGGCTGGAGCCG
GATTATCAAATTACTGTTCCCCGCACATTTAAACCGGAAGGAGTCGAAGATGGATCTGCGTGA

Structures

Toxin

• AlphaFold DB: A0A061KTE0