Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2218737..2219263 Replicon chromosome
Accession NZ_AP022030
Organism Escherichia coli strain WP3-S18-ESBL-07

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag H7R16_RS10715 Protein ID WP_000323025.1
Coordinates 2218976..2219263 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag H7R16_RS10710 Protein ID WP_000534858.1
Coordinates 2218737..2218976 (+) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R16_RS10685 2214465..2214698 - 234 WP_001341330.1 IS66 family insertion sequence element accessory protein TnpB -
H7R16_RS10690 2214746..2214844 - 99 Protein_2085 IS66 family insertion sequence element accessory protein TnpB -
H7R16_RS22550 2215066..2215353 + 288 Protein_2086 hypothetical protein -
H7R16_RS10695 2216122..2217435 + 1314 WP_000589005.1 ISNCY family transposase -
H7R16_RS10700 2217872..2218204 - 333 WP_001301033.1 protein FlxA -
H7R16_RS10705 2218407..2218712 - 306 WP_001326990.1 hypothetical protein -
H7R16_RS10710 2218737..2218976 + 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
H7R16_RS10715 2218976..2219263 + 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
H7R16_RS10720 2219335..2219490 + 156 WP_000813254.1 type I toxin-antitoxin system toxin HokD -
H7R16_RS10725 2219707..2219958 + 252 WP_000980994.1 hypothetical protein -
H7R16_RS10730 2220025..2220303 + 279 WP_012304870.1 hypothetical protein -
H7R16_RS10735 2220305..2221354 + 1050 WP_001265199.1 DUF968 domain-containing protein -
H7R16_RS10740 2221368..2222120 + 753 WP_001047135.1 antitermination protein -
H7R16_RS10745 2222398..2222487 - 90 WP_120795389.1 hypothetical protein -
H7R16_RS10750 2222542..2222754 - 213 WP_000087756.1 cold shock-like protein CspF -
H7R16_RS10755 2223055..2223270 + 216 WP_000066484.1 cold shock-like protein CspB -
H7R16_RS10760 2224024..2224239 + 216 WP_000839590.1 phage lysis protein EssD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 2207952..2234451 26499


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T34153 WP_000323025.1 NZ_AP022030:2218976-2219263 [Escherichia coli]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T34153 NZ_AP022030:2218976-2219263 [Escherichia coli]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT34153 WP_000534858.1 NZ_AP022030:2218737-2218976 [Escherichia coli]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT34153 NZ_AP022030:2218737-2218976 [Escherichia coli]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGTGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References