Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 50121..50764 Replicon plasmid pWP3-S18-ESBL-03_2
Accession NZ_AP022016
Organism Klebsiella quasipneumoniae strain WP3-S18-ESBL-03

Toxin (Protein)


Gene name vagD Uniprot ID D7Y5A6
Locus tag H7R87_RS28050 Protein ID WP_001044765.1
Coordinates 50348..50764 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag H7R87_RS28045 Protein ID WP_001261282.1
Coordinates 50121..50351 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R87_RS28005 45126..45599 + 474 WP_047353183.1 YkgJ family cysteine cluster protein -
H7R87_RS28010 45756..46718 - 963 WP_004197772.1 Abi family protein -
H7R87_RS28015 46885..47667 - 783 WP_004197768.1 site-specific integrase -
H7R87_RS28020 47667..48485 - 819 WP_004197776.1 hypothetical protein -
H7R87_RS28025 48534..48863 - 330 WP_004197773.1 hypothetical protein -
H7R87_RS28030 48891..49199 - 309 WP_004197779.1 hypothetical protein -
H7R87_RS28035 49255..49575 - 321 WP_004197770.1 hypothetical protein -
H7R87_RS28040 49751..50164 - 414 WP_182972096.1 hypothetical protein -
H7R87_RS28045 50121..50351 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H7R87_RS28050 50348..50764 + 417 WP_001044765.1 type II toxin-antitoxin system VapC family toxin Toxin
H7R87_RS28055 50838..51851 + 1014 Protein_60 AAA family ATPase -
H7R87_RS28060 51908..52605 + 698 Protein_61 IS1 family transposase -
H7R87_RS28065 52621..53253 - 633 Protein_62 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..139507 139507


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15148.60 Da        Isoelectric Point: 7.1082

>T34137 WP_001044765.1 NZ_AP022016:50348-50764 [Klebsiella quasipneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLELAVLRGQRIVVSAITYSEMRFGATGPKASPRHVQLVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPDLVLEDWVR

Download         Length: 417 bp

>T34137 NZ_AP022016:50348-50764 [Klebsiella quasipneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCTGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCCAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTGCCGGATCTGGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT34137 WP_001261282.1 NZ_AP022016:50121-50351 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT34137 NZ_AP022016:50121-50351 [Klebsiella quasipneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2H9AY90

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References