Detailed information of TA system TA034136

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 205319..206229
• Replicon: plasmid pWP3-S18-ESBL-03_1
• Accession: NZ_AP022015
• Organism: Klebsiella quasipneumoniae strain WP3-S18-ESBL-03

Toxin (Protein)

• Gene name: -
• Uniprot ID: R4YBC5
• Locus tag: H7R87_RS27045
• Protein ID: WP_004144067.1
• Coordinates: 205319..205789 (-)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A6P1V3Q9
• Locus tag: H7R87_RS27050
• Protein ID: WP_004026357.1
• Coordinates: 205786..206229 (-)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R87_RS27020	200551..200823	-	273	WP_047062072.1	hypothetical protein	-
H7R87_RS27025	201517..201678	-	162	WP_004118124.1	type I toxin-antitoxin system Hok family toxin	-
H7R87_RS27030	201750..202796	+	1047	WP_017145254.1	IS481-like element ISKpn28 family transposase	-
H7R87_RS27040	204948..205208	+	261	WP_004026352.1	hypothetical protein	-
H7R87_RS27045	205319..205789	-	471	WP_004144067.1	RES family NAD+ phosphorylase	Toxin
H7R87_RS27050	205786..206229	-	444	WP_004026357.1	DUF2384 domain-containing protein	Antitoxin
H7R87_RS27055	206330..207601	-	1272	WP_099745241.1	translesion error-prone DNA polymerase V subunit UmuC	-
H7R87_RS27060	207601..208023	-	423	WP_004197884.1	translesion error-prone DNA polymerase V autoproteolytic subunit	-
H7R87_RS27065	208091..209311	-	1221	WP_182972061.1	ISL3-like element ISCfr12 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..346351	346351
	-	inside	IScluster/Tn	-	-	201750..213261	11511

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre-like, helix-turn-helix domain (30-89)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17573.83 Da
• Isoelectric Point: 4.5152

>T34136 WP_004144067.1 NZ_AP022015:c205789-205319 [Klebsiella quasipneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T34136 NZ_AP022015:c205789-205319 [Klebsiella quasipneumoniae]
GTGATCCTCTACAGGCTGACGAAAACAAAGTATCTTTCTACGGCCTGGACCGGATATGGTGCGAAAGAGGCTGGTGGGCG
CTGGAATAGCGTGGGGGTGTCAATGGTATATGTCTCCGAAACGGCATCACTAACGATGCTGGAGACGCTGGTACACCTGC
ATGCGGCACAGATAATGGACTCTTTCACGCTACTGAGTATCGATGTGCCTGATGAACTGATCCAAAGCGCAAACATGGAT
GAATTACCGGACAACTGGGCTGATGAGGATGCGCCTCAGGAACTGGCTGATTACGGAGATGCCTGGTCATTTACCAGGAG
CTCGGTTGCTCTGCGTGTTCCCTCAGCCTTATCGCCGGTAGAGTTCAACTATCTGTTAAATCCTGAGCACCCTGAGTTTT
ACGGGATCGTGCAGAAGGCTCAACAGATACCGTTCCGGTTTGATAGTCGTCTTAAACCTGACAGGAAGTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16480.80 Da
• Isoelectric Point: 10.2498

>AT34136 WP_004026357.1 NZ_AP022015:c206229-205786 [Klebsiella quasipneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT34136 NZ_AP022015:c206229-205786 [Klebsiella quasipneumoniae]
ATGAAAACATTTAGTTTGTCCTCAACTCCAGCCAGACCACAGCGCCTCTGGCAGGTTGCCGGGTTAAATAATGCTGATGG
CGTAGCGCTTCTGGGCCAAATCAATGAAGGCCTGGACGGGAAGGTGGCGAACCGGATCACTGACTGGGCCAGAATAACCC
AGAATGACCTGAGAAAAATGTCCGGTATCCCGTCGACCACGTTTAGCCGCAGTGTGAAGGCACGTTTTAACCCGGAACAA
AGTGAACGTCTGGTAAGGATTATCCGCGTCATTGATCGGGCGGTAGATTTGTTTGAAGGCGACAAGGAAGCCGCACAGAA
GTGGTTGAACGAACCTAACCGGGCATTGAGCTGGAAAGTGCCAGCAGACCTGATGGCATCCGAGACGGGAGCGTATGAGG
TCATTAAGCTGATTACGCGTCTGGAGCATGGGGTGTACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A384IED8

Antitoxin

• AlphaFold DB: A0A6P1V3Q9