Detailed information of TA system TA034135

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 180277..181013
• Replicon: plasmid pWP3-S18-ESBL-03_1
• Accession: NZ_AP022015
• Organism: Klebsiella quasipneumoniae strain WP3-S18-ESBL-03

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: H7R87_RS26865
• Protein ID: WP_003026803.1
• Coordinates: 180531..181013 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: H7R87_RS26860
• Protein ID: WP_003026799.1
• Coordinates: 180277..180543 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R87_RS26825	176121..176483	-	363	WP_004206665.1	arsenite efflux transporter metallochaperone ArsD	-
H7R87_RS26830	176535..176885	-	351	WP_004206664.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
H7R87_RS26835	177243..177491	+	249	WP_004193994.1	hypothetical protein	-
H7R87_RS26840	177488..178699	+	1212	WP_182972058.1	hypothetical protein	-
H7R87_RS26845	178833..179324	+	492	WP_004206662.1	hypothetical protein	-
H7R87_RS26850	179385..179588	+	204	WP_004206661.1	hemolysin expression modulator Hha	-
H7R87_RS26855	179602..179832	+	231	WP_004206660.1	hypothetical protein	-
H7R87_RS26860	180277..180543	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
H7R87_RS26865	180531..181013	+	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
H7R87_RS26870	181214..182617	+	1404	WP_182972059.1	ISNCY-like element ISKpn21 family transposase	-
H7R87_RS26875	182646..183278	-	633	WP_001567369.1	hypothetical protein	-
H7R87_RS26880	183713..184717	-	1005	Protein_191	IS110 family transposase	-
H7R87_RS26885	184796..185353	-	558	WP_003100847.1	recombinase family protein	-
H7R87_RS26890	185347..185718	-	372	WP_003100853.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..346351	346351

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T34135 WP_003026803.1 NZ_AP022015:180531-181013 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T34135 NZ_AP022015:180531-181013 [Klebsiella quasipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGCAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT34135 WP_003026799.1 NZ_AP022015:180277-180543 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT34135 NZ_AP022015:180277-180543 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66