Detailed information of TA system TA034133

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 42251..43002
• Replicon: plasmid pWP3-S18-ESBL-03_1
• Accession: NZ_AP022015
• Organism: Klebsiella quasipneumoniae strain WP3-S18-ESBL-03

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: H7R87_RS26155
• Protein ID: WP_101826042.1
• Coordinates: 42520..43002 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A6S4YWG5
• Locus tag: H7R87_RS26150
• Protein ID: WP_012540170.1
• Coordinates: 42251..42529 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R87_RS26125	37378..38382	+	1005	WP_000427623.1	IS110-like element IS4321 family transposase	-
H7R87_RS26130	38817..39416	+	600	WP_123282530.1	recombinase family protein	-
H7R87_RS26135	39423..39845	+	423	WP_182952522.1	hypothetical protein	-
H7R87_RS26140	40089..40403	-	315	WP_017146616.1	hypothetical protein	-
H7R87_RS26145	41407..41943	-	537	WP_182952510.1	hypothetical protein	-
H7R87_RS26150	42251..42529	+	279	WP_012540170.1	DUF1778 domain-containing protein	Antitoxin
H7R87_RS26155	42520..43002	+	483	WP_101826042.1	GNAT family N-acetyltransferase	Toxin
H7R87_RS26160	43040..43444	+	405	WP_048293664.1	DUF2251 domain-containing protein	-
H7R87_RS26165	43781..44614	+	834	WP_012540133.1	GIY-YIG nuclease family protein	-
H7R87_RS26170	44678..44875	+	198	WP_109037821.1	bacteriocin immunity protein	-
H7R87_RS26175	44970..45206	-	237	WP_012540047.1	hypothetical protein	-
H7R87_RS26180	46251..46979	+	729	WP_182952511.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	pla	1..346351	346351

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (44-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17698.59 Da
• Isoelectric Point: 9.1776

>T34133 WP_101826042.1 NZ_AP022015:42520-43002 [Klebsiella quasipneumoniae]
MGMRPPEPLTPEHNIADFCCQDQVLSEWLKKKALKNHSTGLSRVFVVCAENTNRVIAYYCLASGSVHRNTVPGAYRRNAP
EALPVIVLGRLAVDAAWARKGLGAALLKDAIYRTEHIAIQVGVRALLVHALNDEVREFYTKFGFEPSIANALTLLFPIKT

Nucleotide

• Length: 483 bp

>T34133 NZ_AP022015:42520-43002 [Klebsiella quasipneumoniae]
ATGGGAATGAGGCCTCCGGAACCTCTTACCCCTGAACATAACATTGCTGATTTTTGTTGTCAGGATCAGGTTCTTTCTGA
GTGGTTGAAGAAAAAGGCGCTGAAGAACCATAGCACGGGCCTTTCAAGAGTTTTCGTCGTCTGTGCTGAAAATACGAATA
GGGTAATTGCGTACTACTGTTTGGCATCCGGAAGCGTGCACAGAAACACCGTACCCGGAGCATACCGTCGTAATGCACCA
GAGGCTTTACCGGTCATTGTTCTGGGAAGGCTTGCGGTTGACGCTGCCTGGGCAAGAAAAGGGCTGGGAGCTGCACTGCT
TAAGGATGCGATATACCGAACAGAACATATTGCAATACAGGTTGGTGTTAGGGCGCTTCTTGTTCACGCCTTAAATGATG
AAGTTAGGGAATTTTACACAAAGTTTGGATTCGAGCCCTCCATTGCCAATGCGCTGACATTATTATTTCCGATCAAAACT
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10749.33 Da
• Isoelectric Point: 4.6722

>AT34133 WP_012540170.1 NZ_AP022015:42251-42529 [Klebsiella quasipneumoniae]
MKPDIREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEVACREAQDILLDQRLFLLDDKQFNAFVEELDAPMTPERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT34133 NZ_AP022015:42251-42529 [Klebsiella quasipneumoniae]
ATGAAACCAGACATTCGTGAGGCTCCGATAAACATCCGGGCGAAAACATCTCAACGCGATCTTATCGACATGGCCGCAAA
TCTTGTATCTAAATCACGTACTGATTTTATGCTGGAAGTGGCCTGCCGCGAAGCGCAGGACATCTTGTTAGATCAGCGCT
TGTTTCTGCTGGATGATAAGCAGTTTAACGCGTTTGTCGAAGAGCTTGACGCCCCTATGACCCCAGAGCGTCAGGCGCGT
ATTGATAACCTGATGAACCGCAAATCACCATGGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6S4YWG5