Detailed information of TA system TA034115

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/ElaA-DUF1778
• Location: 13996..14756
• Replicon: plasmid pWP3-S18-CRE-02_4
• Accession: NZ_AP022009
• Organism: Klebsiella quasipneumoniae strain WP3-S18-CRE-02

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: H7R71_RS28750
• Protein ID: WP_064402685.1
• Coordinates: 14271..14756 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: -
• Locus tag: H7R71_RS28745
• Protein ID: WP_086531840.1
• Coordinates: 13996..14283 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R71_RS28715	9034..9864	+	831	Protein_10	ISNCY family transposase	-
H7R71_RS28720	10038..10205	-	168	Protein_11	phage baseplate assembly protein V	-
H7R71_RS28725	10291..11004	+	714	WP_064402460.1	hypothetical protein	-
H7R71_RS28730	11277..12353	-	1077	WP_064402463.1	hypothetical protein	-
H7R71_RS28735	12509..12631	-	123	Protein_14	IS481 family transposase	-
H7R71_RS28740	12734..13894	+	1161	WP_076752093.1	IS30 family transposase	-
H7R71_RS28745	13996..14283	+	288	WP_086531840.1	DUF1778 domain-containing protein	Antitoxin
H7R71_RS28750	14271..14756	+	486	WP_064402685.1	GNAT family N-acetyltransferase	Toxin
H7R71_RS28755	15053..16126	-	1074	WP_182949654.1	IS481-like element ISKpn28 family transposase	-
H7R71_RS28760	16406..17251	+	846	Protein_19	LysR family transcriptional regulator	-
H7R71_RS28765	17304..18008	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
H7R71_RS28770	18076..18882	-	807	Protein_21	type I restriction endonuclease subunit R	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..55811	55811
	-	inside	IScluster/Tn	-	-	5420..18008	12588
	-	inside	Integron	-	-	12734..14756	2022

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (13-89)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17791.51 Da
• Isoelectric Point: 9.4841

>T34115 WP_064402685.1 NZ_AP022009:14271-14756 [Klebsiella quasipneumoniae]
VGRVTAPEPLSPYHQVAEFVSGETVLDDWLKQKGLKNQALGAARTFVVCKKDTQQIAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSQTQERTLFLKLP
Q

Nucleotide

• Length: 486 bp

>T34115 NZ_AP022009:14271-14756 [Klebsiella quasipneumoniae]
GTGGGACGTGTAACAGCACCAGAACCTCTTTCCCCTTATCATCAGGTGGCTGAGTTCGTCAGTGGTGAGACTGTGCTCGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGAGCGGCCCGAACGTTTGTGGTGTGTAAGAAGGACACTC
AGCAAATTGCTGGTTTTTACTCACTGGCAACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGCCGTAACATG
CCAGATCCCATTCCTGTCATTATACTAGCCCGTCTTGCCGTCGATCTCTCATTCCGCGGGAAAGGGCTTGGCGCTGATTT
ACTTCATGACGCAGTGCTTCGTTGCTATCGTGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCGAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGGAACGGACATTATTCCTTAAACTCCCT
CAATAA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 11084.82 Da
• Isoelectric Point: 4.6696

>AT34115 WP_086531840.1 NZ_AP022009:13996-14283 [Klebsiella quasipneumoniae]
MLYKRCLMKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVTDD
PVIEKLLVRKPQWDV

Nucleotide

• Length: 288 bp

>AT34115 NZ_AP022009:13996-14283 [Klebsiella quasipneumoniae]
ATGCTATACAAGAGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAATGGCCTGTCAGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGCGGAGTTCATCGATATGCTTGATGCACCCGTTACAGATGAT
CCCGTTATCGAAAAACTGCTGGTAAGGAAACCTCAGTGGGACGTGTAA