Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 100635..101371 Replicon plasmid pWP3-S18-CRE-02_1
Accession NZ_AP022006
Organism Klebsiella quasipneumoniae strain WP3-S18-CRE-02

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag H7R71_RS27150 Protein ID WP_003026803.1
Coordinates 100889..101371 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag H7R71_RS27145 Protein ID WP_003026799.1
Coordinates 100635..100901 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R71_RS27110 96479..96841 - 363 WP_004206665.1 arsenite efflux transporter metallochaperone ArsD -
H7R71_RS27115 96893..97243 - 351 WP_004206664.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
H7R71_RS27120 97601..97849 + 249 WP_004193994.1 hypothetical protein -
H7R71_RS27125 97846..99057 + 1212 WP_032415728.1 hypothetical protein -
H7R71_RS27130 99191..99682 + 492 WP_004206662.1 hypothetical protein -
H7R71_RS27135 99743..99946 + 204 WP_004206661.1 hemolysin expression modulator Hha -
H7R71_RS27140 99960..100190 + 231 WP_004206660.1 hypothetical protein -
H7R71_RS27145 100635..100901 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
H7R71_RS27150 100889..101371 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
H7R71_RS27155 101572..102975 + 1404 WP_001567368.1 ISNCY-like element ISKpn21 family transposase -
H7R71_RS27160 103004..103636 - 633 WP_001567369.1 hypothetical protein -
H7R71_RS27165 104160..105164 - 1005 WP_182949562.1 IS110-like element IS4321 family transposase -
H7R71_RS27170 105243..105800 - 558 WP_003100847.1 recombinase family protein -
H7R71_RS27175 105794..106165 - 372 WP_003100853.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..143641 143641


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T34114 WP_003026803.1 NZ_AP022006:100889-101371 [Klebsiella quasipneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T34114 NZ_AP022006:100889-101371 [Klebsiella quasipneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT34114 WP_003026799.1 NZ_AP022006:100635-100901 [Klebsiella quasipneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT34114 NZ_AP022006:100635-100901 [Klebsiella quasipneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATTCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References