Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 53802..54445 Replicon plasmid pWP3-S18-CRE-02_1
Accession NZ_AP022006
Organism Klebsiella quasipneumoniae strain WP3-S18-CRE-02

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag H7R71_RS26890 Protein ID WP_001044770.1
Coordinates 54029..54445 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag H7R71_RS26885 Protein ID WP_001261282.1
Coordinates 53802..54032 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R71_RS26850 49440..50408 + 969 WP_000654811.1 IS5 family transposase -
H7R71_RS26855 50726..51751 - 1026 WP_001101446.1 IS110 family transposase -
H7R71_RS26860 51971..52186 - 216 WP_074186229.1 membrane protein insertion efficiency factor YidD -
H7R71_RS26865 52187..52432 - 246 WP_064158658.1 hypothetical protein -
H7R71_RS26870 52690..52995 - 306 WP_006788214.1 type II toxin-antitoxin system toxin CcdB -
H7R71_RS26875 52997..53215 - 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA -
H7R71_RS26880 53385..53845 - 461 Protein_61 hypothetical protein -
H7R71_RS26885 53802..54032 + 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H7R71_RS26890 54029..54445 + 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
H7R71_RS26895 54519..56081 + 1563 WP_004206609.1 AAA family ATPase -
H7R71_RS26900 56066..57088 + 1023 WP_000361404.1 helicase UvrD -
H7R71_RS26905 57626..58540 + 915 WP_031592169.1 HNH endonuclease -
H7R71_RS26910 58726..59076 - 351 WP_004187110.1 DUF305 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..143641 143641


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T34113 WP_001044770.1 NZ_AP022006:54029-54445 [Klebsiella quasipneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T34113 NZ_AP022006:54029-54445 [Klebsiella quasipneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT34113 WP_001261282.1 NZ_AP022006:53802-54032 [Klebsiella quasipneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT34113 NZ_AP022006:53802-54032 [Klebsiella quasipneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References