Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 2146..2672 Replicon plasmid pWP3-S18-CRE-02_1
Accession NZ_AP022006
Organism Klebsiella quasipneumoniae strain WP3-S18-CRE-02

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag H7R71_RS26590 Protein ID WP_000323025.1
Coordinates 2146..2433 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID S1F6D3
Locus tag H7R71_RS26595 Protein ID WP_000534858.1
Coordinates 2433..2672 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R71_RS26575 101..958 - 858 WP_013023768.1 incFII family plasmid replication initiator RepA -
H7R71_RS26580 951..998 - 48 WP_073984493.1 hypothetical protein -
H7R71_RS26585 1061..2257 + 1197 WP_182949576.1 IS481 family transposase -
H7R71_RS26590 2146..2433 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
H7R71_RS26595 2433..2672 - 240 WP_000534858.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
H7R71_RS26600 2935..3858 - 924 WP_020277922.1 cation diffusion facilitator family transporter -
H7R71_RS26605 3933..4913 + 981 WP_000019450.1 IS5-like element ISKpn26 family transposase -
H7R71_RS26610 5061..5432 - 372 Protein_7 cytochrome b/b6 domain-containing protein -
H7R71_RS26615 5980..6960 - 981 WP_014386491.1 IS5-like element ISKpn26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..143641 143641
- inside IScluster/Tn - - 1061..13573 12512


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T34111 WP_000323025.1 NZ_AP022006:c2433-2146 [Klebsiella quasipneumoniae]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T34111 NZ_AP022006:c2433-2146 [Klebsiella quasipneumoniae]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9071.48 Da        Isoelectric Point: 4.5230

>AT34111 WP_000534858.1 NZ_AP022006:c2672-2433 [Klebsiella quasipneumoniae]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRNPKPVRVTLDEL

Download         Length: 240 bp

>AT34111 NZ_AP022006:c2672-2433 [Klebsiella quasipneumoniae]
ATGGGTAGCATTAACCTACGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGACTTCGTAATCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
PDB 4FXE
PDB 2KC8
PDB 2K29
AlphaFold DB A0A4V1CTS8

References