Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-YafN
Location 12198..12733 Replicon plasmid pWP3-W18-ESBL-08_1
Accession NZ_AP022004
Organism Escherichia coli strain WP3-W18-ESBL-08

Toxin (Protein)


Gene name relE Uniprot ID A0A0M1UJC2
Locus tag H7R22_RS25210 Protein ID WP_001270422.1
Coordinates 12198..12485 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID L4J0V6
Locus tag H7R22_RS25215 Protein ID WP_001132895.1
Coordinates 12482..12733 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R22_RS25185 7336..8505 - 1170 WP_001072358.1 hypothetical protein -
H7R22_RS25190 8872..9060 + 189 WP_001332784.1 hypothetical protein -
H7R22_RS25195 9181..9921 + 741 WP_001066947.1 tyrosine-type recombinase/integrase -
H7R22_RS25200 10164..11141 - 978 WP_001309253.1 RepB family plasmid replication initiator protein -
H7R22_RS25205 11785..12196 - 412 Protein_14 DDE-type integrase/transposase/recombinase -
H7R22_RS25210 12198..12485 - 288 WP_001270422.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H7R22_RS25215 12482..12733 - 252 WP_001132895.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
H7R22_RS25220 13491..14696 + 1206 WP_000817635.1 AAA family ATPase -
H7R22_RS25225 14693..15655 + 963 WP_000756328.1 ParB/RepB/Spo0J family partition protein -
H7R22_RS25230 15889..16665 + 777 Protein_19 hypothetical protein -
H7R22_RS25235 16832..16975 + 144 WP_000270807.1 DUF1281 domain-containing protein -
H7R22_RS25240 16982..17341 + 360 WP_001336695.1 DUF1281 domain-containing protein -
H7R22_RS25245 17405..17716 + 312 WP_001309256.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - senB 1..131334 131334
- flank IS/Tn - - 16312..16452 140


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11262.30 Da        Isoelectric Point: 10.4703

>T34090 WP_001270422.1 NZ_AP022004:c12485-12198 [Escherichia coli]
MRYQVKFREDALKEWQKMDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Download         Length: 288 bp

>T34090 NZ_AP022004:c12485-12198 [Escherichia coli]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAAATGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGCGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTGGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAA
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9178.47 Da        Isoelectric Point: 4.2108

>AT34090 WP_001132895.1 NZ_AP022004:c12733-12482 [Escherichia coli]
MPNIILSDTSASVSELKKNPMATVSAGDGFPVAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Download         Length: 252 bp

>AT34090 NZ_AP022004:c12733-12482 [Escherichia coli]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGGTCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTGGAT
AAATACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M1UJC2

Antitoxin

Source ID Structure
AlphaFold DB L4J0V6

References