Detailed information of TA system TA034087

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4725085..4725919
• Replicon: chromosome
• Accession: NZ_AP022003
• Organism: Escherichia coli strain WP3-W18-ESBL-08

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: B7MK10
• Locus tag: H7R22_RS22830
• Protein ID: WP_000854820.1
• Coordinates: 4725542..4725919 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B7MK11
• Locus tag: H7R22_RS22825
• Protein ID: WP_001285610.1
• Coordinates: 4725085..4725453 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R22_RS22790	4720806..4721486	+	681	WP_021515201.1	WYL domain-containing protein	-
H7R22_RS22795	4721637..4722314	+	678	WP_060579144.1	hypothetical protein	-
H7R22_RS22800	4722320..4722553	+	234	WP_000883181.1	DUF905 family protein	-
H7R22_RS22805	4722643..4723461	+	819	WP_001175155.1	DUF945 domain-containing protein	-
H7R22_RS22810	4723727..4724206	+	480	WP_000706978.1	antirestriction protein	-
H7R22_RS22815	4724221..4724697	+	477	WP_001186193.1	RadC family protein	-
H7R22_RS22820	4724784..4725005	+	222	WP_000692347.1	DUF987 domain-containing protein	-
H7R22_RS22825	4725085..4725453	+	369	WP_001285610.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
H7R22_RS22830	4725542..4725919	+	378	WP_000854820.1	type IV toxin-antitoxin system toxin CbtA	Toxin
H7R22_RS22835	4725916..4726113	+	198	Protein_4472	hypothetical protein	-
H7R22_RS22840	4726141..4726338	+	198	WP_085949158.1	DUF957 domain-containing protein	-
H7R22_RS22845	4726423..4726983	+	561	Protein_4474	DUF4942 domain-containing protein	-
H7R22_RS22850	4727818..4729356	+	1539	WP_001187178.1	lysine decarboxylation/transport transcriptional activator CadC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4691795..4737169	45374

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (19-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14043.12 Da
• Isoelectric Point: 7.8839

>T34087 WP_000854820.1 NZ_AP022003:4725542-4725919 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNIILGKHPEVKQ

Nucleotide

• Length: 378 bp

>T34087 NZ_AP022003:4725542-4725919 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAATATTATCCTGGGTAAGCACCCGGAGGTGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13606.39 Da
• Isoelectric Point: 6.0618

>AT34087 WP_001285610.1 NZ_AP022003:4725085-4725453 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADSYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT34087 NZ_AP022003:4725085-4725453 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCTTCCCGACGACAATAACGACCGTACCTGGTGGGGACTGCCCTGCACCGTGAC
GCCCTGTTTTGGTGCCCGTCTGGTACAGGAAGGCAACCGGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCGGT
TCAGCGACGCGGATTCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A0E2KXX6