Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 4546311..4546833 Replicon chromosome
Accession NZ_AP022003
Organism Escherichia coli strain WP3-W18-ESBL-08

Toxin (Protein)


Gene name relE Uniprot ID A0A829L6G8
Locus tag H7R22_RS21885 Protein ID WP_001105433.1
Coordinates 4546311..4546601 (-) Length 97 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SC69
Locus tag H7R22_RS21890 Protein ID WP_000212715.1
Coordinates 4546591..4546833 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R22_RS21870 4541479..4543134 + 1656 WP_001550509.1 alpha,alpha-phosphotrehalase -
H7R22_RS21875 4543528..4545666 + 2139 WP_000187798.1 anaerobic ribonucleoside-triphosphate reductase -
H7R22_RS21880 4545846..4546310 + 465 WP_001009182.1 anaerobic ribonucleoside-triphosphate reductase-activating protein -
H7R22_RS21885 4546311..4546601 - 291 WP_001105433.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
H7R22_RS21890 4546591..4546833 - 243 WP_000212715.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
H7R22_RS21895 4547025..4547411 - 387 WP_001232246.1 cytochrome b562 -
H7R22_RS21900 4547594..4548946 - 1353 WP_001162173.1 metalloprotease PmbA -
H7R22_RS21905 4549040..4549591 + 552 WP_000166270.1 ribosome-associated protein -
H7R22_RS21910 4549741..4551114 - 1374 WP_001522402.1 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-83)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-72)


Sequences

Toxin        


Download         Length: 97 a.a.        Molecular weight: 11367.23 Da        Isoelectric Point: 10.0238

>T34085 WP_001105433.1 NZ_AP022003:c4546601-4546311 [Escherichia coli]
MNYELAFDPRALKEWQKLGVTVREQFKKKLEDVLKRPRNPSAKLRDLPDCYKIKLRTQGYRLVYQVNDKELLVLVIAIGK
RENSAVYEDATKRLDE

Download         Length: 291 bp

>T34085 NZ_AP022003:c4546601-4546311 [Escherichia coli]
ATGAACTATGAGCTGGCGTTTGATCCGCGGGCGCTGAAAGAGTGGCAAAAACTGGGCGTGACAGTCCGGGAACAATTTAA
AAAAAAGCTTGAGGATGTGCTGAAAAGGCCGCGCAATCCATCGGCTAAACTGCGGGATTTACCGGACTGCTACAAAATTA
AGCTGCGCACACAGGGATATCGCCTGGTTTATCAGGTTAACGATAAAGAGTTGCTCGTGTTGGTGATAGCTATTGGTAAG
AGAGAGAACTCAGCCGTTTATGAAGACGCGACTAAACGGCTGGATGAGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9125.56 Da        Isoelectric Point: 5.1369

>AT34085 WP_000212715.1 NZ_AP022003:c4546833-4546591 [Escherichia coli]
MATLNVRLDDKLKNEAYAVLEKLNITPTEAVRLLFQYVAENGRMPVKTLTVSDSEEALLRTVRERLANPQEAIKVRLDEL

Download         Length: 243 bp

>AT34085 NZ_AP022003:c4546833-4546591 [Escherichia coli]
ATGGCCACACTTAACGTCCGCCTGGATGACAAACTGAAAAATGAGGCTTATGCGGTGCTGGAAAAATTAAATATCACGCC
TACTGAAGCAGTGCGGCTGCTGTTTCAGTACGTTGCGGAAAATGGACGAATGCCGGTAAAAACGCTCACTGTCAGTGATA
GTGAAGAAGCCTTGTTACGCACTGTCAGGGAACGACTGGCCAACCCACAAGAGGCCATTAAGGTCCGACTGGATGAACTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829L6G8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9H4B3

References