Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3821477..3822095 Replicon chromosome
Accession NZ_AP022003
Organism Escherichia coli strain WP3-W18-ESBL-08

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H7R22_RS18450 Protein ID WP_001291435.1
Coordinates 3821877..3822095 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H7R22_RS18445 Protein ID WP_000344800.1
Coordinates 3821477..3821851 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R22_RS18435 3816566..3817759 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H7R22_RS18440 3817782..3820931 + 3150 WP_001132480.1 multidrug efflux RND transporter permease subunit -
H7R22_RS18445 3821477..3821851 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H7R22_RS18450 3821877..3822095 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
H7R22_RS18455 3822267..3822818 + 552 WP_060579167.1 maltose O-acetyltransferase -
H7R22_RS18460 3822934..3823404 + 471 WP_000136192.1 YlaC family protein -
H7R22_RS18465 3823568..3825118 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H7R22_RS18470 3825160..3825513 - 354 WP_001550741.1 DUF1428 family protein -
H7R22_RS18480 3825892..3826203 + 312 WP_000409911.1 MGMT family protein -
H7R22_RS18485 3826234..3826806 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T34080 WP_001291435.1 NZ_AP022003:3821877-3822095 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T34080 NZ_AP022003:3821877-3822095 [Escherichia coli]
ATGTCCGAAAAACCTTTAACAAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT34080 WP_000344800.1 NZ_AP022003:3821477-3821851 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT34080 NZ_AP022003:3821477-3821851 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGCAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References