Detailed information of TA system TA034079

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3335230..3335935
• Replicon: chromosome
• Accession: NZ_AP022003
• Organism: Escherichia coli strain WP3-W18-ESBL-08

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: H7R22_RS16110
• Protein ID: WP_000539521.1
• Coordinates: 3335230..3335616 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: H7R22_RS16115
• Protein ID: WP_001280945.1
• Coordinates: 3335606..3335935 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R22_RS16090	3331234..3331860	+	627	WP_001295292.1	glutathione S-transferase GstB	-
H7R22_RS16095	3331857..3332972	-	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
H7R22_RS16100	3333083..3333466	-	384	WP_000497137.1	biofilm formation regulator BssR	-
H7R22_RS16105	3333679..3335004	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
H7R22_RS16110	3335230..3335616	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
H7R22_RS16115	3335606..3335935	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
H7R22_RS16120	3336005..3337333	-	1329	WP_001550887.1	GGDEF domain-containing protein	-
H7R22_RS16125	3337341..3339104	-	1764	Protein_3161	EAL domain-containing protein	-
H7R22_RS16130	3339120..3339817	-	698	Protein_3162	IS1-like element IS1A family transposase	-
H7R22_RS16135	3339846..3340466	-	621	WP_182914561.1	CHASE9 sensor domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T34079 WP_000539521.1 NZ_AP022003:3335230-3335616 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T34079 NZ_AP022003:3335230-3335616 [Escherichia coli]
ATGGGTGTGTATAAGGCAAGACGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAGGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGTTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCGGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTTTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT34079 WP_001280945.1 NZ_AP022003:3335606-3335935 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT34079 NZ_AP022003:3335606-3335935 [Escherichia coli]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCACTGGGCCTGATGTCTGATAGTGAAGTTGA
TGCCATTGCCGTCATGGTCAAAGCTCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATCA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCATTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6