Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 36573..37098 Replicon plasmid pWP3-W18-ESBL-07_1
Accession NZ_AP021999
Organism Escherichia coli strain WP3-W18-ESBL-07

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag H7Q99_RS24415 Protein ID WP_001159868.1
Coordinates 36793..37098 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag H7Q99_RS24410 Protein ID WP_000813634.1
Coordinates 36573..36791 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7Q99_RS24375 31674..32033 - 360 WP_001513660.1 hypothetical protein -
H7Q99_RS24380 32061..32240 - 180 WP_001513661.1 hypothetical protein -
H7Q99_RS24385 32245..32625 - 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin -
H7Q99_RS24390 32625..32846 - 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
H7Q99_RS24395 33100..33797 + 698 Protein_37 IS1 family transposase -
H7Q99_RS24400 33934..34866 - 933 WP_000991832.1 hypothetical protein -
H7Q99_RS24405 34870..35865 - 996 WP_000246636.1 hypothetical protein -
H7Q99_RS24410 36573..36791 + 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H7Q99_RS24415 36793..37098 + 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
H7Q99_RS24420 37099..37905 + 807 WP_000016982.1 site-specific integrase -
H7Q99_RS24425 38679..39434 + 756 WP_000852146.1 replication initiation protein RepE -
H7Q99_RS24430 40022..41188 + 1167 WP_000772446.1 plasmid-partitioning protein SopA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaCTX-M-27 senB 1..105864 105864
- inside IScluster/Tn - - 26339..33797 7458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T34054 WP_001159868.1 NZ_AP021999:36793-37098 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T34054 NZ_AP021999:36793-37098 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT34054 WP_000813634.1 NZ_AP021999:36573-36791 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT34054 NZ_AP021999:36573-36791 [Escherichia coli]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References