Detailed information of TA system TA034053

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 32245..32846
• Replicon: plasmid pWP3-W18-ESBL-07_1
• Accession: NZ_AP021999
• Organism: Escherichia coli strain WP3-W18-ESBL-07

Toxin (Protein)

• Gene name: doc
• Uniprot ID: V0AJ64
• Locus tag: H7Q99_RS24385
• Protein ID: WP_001216034.1
• Coordinates: 32245..32625 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: H7Q99_RS24390
• Protein ID: WP_001190712.1
• Coordinates: 32625..32846 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7Q99_RS24350	27296..28228	-	933	WP_000081352.1	homocysteine S-methyltransferase	-
H7Q99_RS24355	28215..29618	-	1404	WP_001373486.1	S-methylmethionine permease	-
H7Q99_RS24360	29826..30842	+	1017	WP_012372828.1	IS5-like element IS5 family transposase	-
H7Q99_RS24365	31070..31387	+	318	WP_001513659.1	hypothetical protein	-
H7Q99_RS24370	31337..31750	-	414	Protein_32	DDE-type integrase/transposase/recombinase	-
H7Q99_RS24375	31674..32033	-	360	WP_001513660.1	hypothetical protein	-
H7Q99_RS24380	32061..32240	-	180	WP_001513661.1	hypothetical protein	-
H7Q99_RS24385	32245..32625	-	381	WP_001216034.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
H7Q99_RS24390	32625..32846	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
H7Q99_RS24395	33100..33797	+	698	Protein_37	IS1 family transposase	-
H7Q99_RS24400	33934..34866	-	933	WP_000991832.1	hypothetical protein	-
H7Q99_RS24405	34870..35865	-	996	WP_000246636.1	hypothetical protein	-
H7Q99_RS24410	36573..36791	+	219	WP_000813634.1	type II toxin-antitoxin system antitoxin CcdA	-
H7Q99_RS24415	36793..37098	+	306	WP_001159868.1	type II toxin-antitoxin system toxin CcdB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	blaCTX-M-27	senB	1..105864	105864
	-	inside	IScluster/Tn	-	-	26339..33797	7458

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13615.32 Da
• Isoelectric Point: 5.1514

>T34053 WP_001216034.1 NZ_AP021999:c32625-32245 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Nucleotide

• Length: 381 bp

>T34053 NZ_AP021999:c32625-32245 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCGGGAATGTC
TGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACAGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT34053 WP_001190712.1 NZ_AP021999:c32846-32625 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT34053 NZ_AP021999:c32846-32625 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAGGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: V0AJ64

Antitoxin

• AlphaFold DB: A0A829CJB6