34046

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 4049659..4050179 Replicon chromosome
Accession NZ_AP021998
Organism Escherichia coli strain WP3-W18-ESBL-07

Toxin (Protein)


Gene name ccdB Uniprot ID A0A061KX66
Locus tag H7Q99_RS19615 Protein ID WP_001160964.1
Coordinates 4049659..4049943 (-) Length 95 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag H7Q99_RS19620 Protein ID WP_000125566.1
Coordinates 4049946..4050179 (-) Length 78 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7Q99_RS19590 4045304..4046590 + 1287 WP_000800457.1 peptidylprolyl isomerase SurA -
H7Q99_RS19595 4046590..4047579 + 990 WP_000241227.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
H7Q99_RS19600 4047576..4048397 + 822 WP_001065365.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
H7Q99_RS19605 4048400..4048777 + 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
H7Q99_RS19610 4048784..4049632 + 849 WP_000257181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
H7Q99_RS19615 4049659..4049943 - 285 WP_001160964.1 CcdB family protein Toxin
H7Q99_RS19620 4049946..4050179 - 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
H7Q99_RS19625 4050265..4050744 - 480 WP_000624375.1 type 3 dihydrofolate reductase -
H7Q99_RS19630 4050936..4052798 - 1863 WP_000377106.1 glutathione-regulated potassium-efflux system protein KefC -
H7Q99_RS19635 4052791..4053321 - 531 WP_000600725.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
H7Q99_RS19640 4053429..4054760 - 1332 WP_001183209.1 MFS transporter -
H7Q99_RS19645 4054819..4055106 - 288 WP_000203744.1 ferredoxin-like protein FixX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-93)

Antitoxin

Post-segregation antitoxin CcdA

(8-77)


Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10692.54 Da        Isoelectric Point: 10.3030

>T34046 WP_001160964.1 NZ_AP021998:c4049943-4049659 [Escherichia coli]
MQFTVYRSRGRNAAFPFVIDVTSDIIGEINRRIVIPLTPIERFSRIRPPERLNPILLLIDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIK

Download         Length: 285 bp

>T34046 NZ_AP021998:c4049943-4049659 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGATAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAATGA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT34046 WP_000125566.1 NZ_AP021998:c4050179-4049946 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT34046 NZ_AP021998:c4050179-4049946 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A061KX66

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References