Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3597973..3598591 Replicon chromosome
Accession NZ_AP021998
Organism Escherichia coli strain WP3-W18-ESBL-07

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag H7Q99_RS17470 Protein ID WP_001291435.1
Coordinates 3598373..3598591 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag H7Q99_RS17465 Protein ID WP_000344800.1
Coordinates 3597973..3598347 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7Q99_RS17455 3593062..3594255 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
H7Q99_RS17460 3594278..3597427 + 3150 WP_001132478.1 multidrug efflux RND transporter permease subunit -
H7Q99_RS17465 3597973..3598347 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
H7Q99_RS17470 3598373..3598591 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
H7Q99_RS17475 3598765..3599316 + 552 WP_000102539.1 maltose O-acetyltransferase -
H7Q99_RS17480 3599432..3599902 + 471 WP_000136192.1 YlaC family protein -
H7Q99_RS17485 3600066..3601616 + 1551 WP_001385227.1 cyclic-guanylate-specific phosphodiesterase PdeB -
H7Q99_RS17490 3601658..3602011 - 354 WP_000878135.1 DUF1428 family protein -
H7Q99_RS17500 3602390..3602701 + 312 WP_000409908.1 MGMT family protein -
H7Q99_RS17505 3602732..3603304 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T34044 WP_001291435.1 NZ_AP021998:3598373-3598591 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T34044 NZ_AP021998:3598373-3598591 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT34044 WP_000344800.1 NZ_AP021998:3597973-3598347 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT34044 NZ_AP021998:3597973-3598347 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References