Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 24404..24929 Replicon plasmid pWP3-W18-ESBL-06_6
Accession NZ_AP021989
Organism Raoultella ornithinolytica strain WP3-W18-ESBL-06

Toxin (Protein)


Gene name ccdB Uniprot ID A0A6S4YF27
Locus tag H7R89_RS30215 Protein ID WP_025714517.1
Coordinates 24624..24929 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag H7R89_RS30210 Protein ID WP_001568025.1
Coordinates 24404..24622 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R89_RS30195 21164..21556 - 393 Protein_19 IS630 family transposase -
H7R89_RS30200 21590..22606 + 1017 WP_001310555.1 IS5-like element IS5 family transposase -
H7R89_RS30205 22837..23811 + 975 WP_182952550.1 sensor domain-containing diguanylate cyclase -
H7R89_RS30210 24404..24622 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
H7R89_RS30215 24624..24929 + 306 WP_025714517.1 type II toxin-antitoxin system toxin CcdB Toxin
H7R89_RS30220 25098..25493 + 396 WP_017899885.1 hypothetical protein -
H7R89_RS30225 25520..25834 + 315 WP_053389906.1 hypothetical protein -
H7R89_RS30230 25845..26861 + 1017 WP_017899884.1 hypothetical protein -
H7R89_RS30235 27059..27853 + 795 WP_004197635.1 site-specific integrase -
H7R89_RS30240 28325..28627 - 303 WP_071571079.1 hypothetical protein -
H7R89_RS30245 28624..29250 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..53995 53995
- flank IS/Tn - - 21626..22606 980


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11562.25 Da        Isoelectric Point: 5.6906

>T34026 WP_025714517.1 NZ_AP021989:24624-24929 [Raoultella ornithinolytica]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T34026 NZ_AP021989:24624-24929 [Raoultella ornithinolytica]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT34026 WP_001568025.1 NZ_AP021989:24404-24622 [Raoultella ornithinolytica]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT34026 NZ_AP021989:24404-24622 [Raoultella ornithinolytica]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6S4YF27

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References