Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 56143..56786 Replicon plasmid pWP3-W18-ESBL-06_3
Accession NZ_AP021986
Organism Raoultella ornithinolytica strain WP3-W18-ESBL-06

Toxin (Protein)


Gene name vagD Uniprot ID A0A7D6UII7
Locus tag H7R89_RS29005 Protein ID WP_032425563.1
Coordinates 56370..56786 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag H7R89_RS29000 Protein ID WP_001261276.1
Coordinates 56143..56373 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
H7R89_RS28970 52337..52450 + 114 WP_014343462.1 hypothetical protein -
H7R89_RS28975 52579..52836 + 258 WP_000764642.1 hypothetical protein -
H7R89_RS28980 52894..53673 - 780 WP_001568028.1 site-specific integrase -
H7R89_RS28985 53857..54834 - 978 WP_182952533.1 hypothetical protein -
H7R89_RS28990 55021..55326 - 306 WP_013023785.1 type II toxin-antitoxin system toxin CcdB -
H7R89_RS28995 55328..55546 - 219 WP_004197642.1 type II toxin-antitoxin system antitoxin CcdA -
H7R89_RS29000 56143..56373 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
H7R89_RS29005 56370..56786 + 417 WP_032425563.1 type II toxin-antitoxin system VapC family toxin Toxin
H7R89_RS29010 56827..57705 - 879 WP_009309916.1 restriction endonuclease -
H7R89_RS29015 58367..59644 + 1278 WP_009309915.1 HlyD family secretion protein -
H7R89_RS29020 59634..61742 + 2109 WP_032427226.1 peptidase domain-containing ABC transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..99872 99872


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15080.56 Da        Isoelectric Point: 8.5403

>T34025 WP_032425563.1 NZ_AP021986:56370-56786 [Raoultella ornithinolytica]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNTREFERVPGLVLEDWVK

Download         Length: 417 bp

>T34025 NZ_AP021986:56370-56786 [Raoultella ornithinolytica]
ATGAAGAAAACCTGGATGCTCGACACTAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAACAGGCGGTGCTGCGCGGTGATCGCATCGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCATCCTGCCCTGGGACCGGGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCTATCGCGGCCGGCGCCATCCTGGTGACGAATAATACAAGAGAGTTTGAGCGGGTGCCGGGTCTGGTGCTGG
AAGACTGGGTGAAGTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT34025 WP_001261276.1 NZ_AP021986:56143-56373 [Raoultella ornithinolytica]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT34025 NZ_AP021986:56143-56373 [Raoultella ornithinolytica]
ATGCGCACAGTTTCGATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGACGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7D6UII7

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References