Detailed information of TA system TA034024

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 55021..55546
• Replicon: plasmid pWP3-W18-ESBL-06_3
• Accession: NZ_AP021986
• Organism: Raoultella ornithinolytica strain WP3-W18-ESBL-06

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D4HQE7
• Locus tag: H7R89_RS28990
• Protein ID: WP_013023785.1
• Coordinates: 55021..55326 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A9E1GDP8
• Locus tag: H7R89_RS28995
• Protein ID: WP_004197642.1
• Coordinates: 55328..55546 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
H7R89_RS28960	50227..50529	+	303	Protein_60	hypothetical protein	-
H7R89_RS28965	50951..51706	-	756	WP_001568031.1	replication initiation protein RepE	-
H7R89_RS28970	52337..52450	+	114	WP_014343462.1	hypothetical protein	-
H7R89_RS28975	52579..52836	+	258	WP_000764642.1	hypothetical protein	-
H7R89_RS28980	52894..53673	-	780	WP_001568028.1	site-specific integrase	-
H7R89_RS28985	53857..54834	-	978	WP_182952533.1	hypothetical protein	-
H7R89_RS28990	55021..55326	-	306	WP_013023785.1	type II toxin-antitoxin system toxin CcdB	Toxin
H7R89_RS28995	55328..55546	-	219	WP_004197642.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
H7R89_RS29000	56143..56373	+	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
H7R89_RS29005	56370..56786	+	417	WP_032425563.1	type II toxin-antitoxin system VapC family toxin	-
H7R89_RS29010	56827..57705	-	879	WP_009309916.1	restriction endonuclease	-
H7R89_RS29015	58367..59644	+	1278	WP_009309915.1	HlyD family secretion protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..99872	99872

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11615.30 Da
• Isoelectric Point: 6.4672

>T34024 WP_013023785.1 NZ_AP021986:c55326-55021 [Raoultella ornithinolytica]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T34024 NZ_AP021986:c55326-55021 [Raoultella ornithinolytica]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCCGTGAACTCTATCCGGTGG
TGCACATCGGGGACGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACTTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8376.34 Da
• Isoelectric Point: 4.7405

>AT34024 WP_004197642.1 NZ_AP021986:c55546-55328 [Raoultella ornithinolytica]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT34024 NZ_AP021986:c55546-55328 [Raoultella ornithinolytica]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A2J4ZZW6